The invention belongs to the field of materials science and engineering and biological separation engineering and particularly to a preparation method for quantum dot-based protein core-shell imprinted microspheres. The preparation method for the quantum dot-based protein core-shell imprinted microspheres comprises the following steps : the improved method of Stober is adopted, so as to synthesize silicon dioxide nano particles with amino on the surface; meanwhile, CdTe quantum dots with carboxyl on the surface are synthesized; the quantum dots are grafted to the silicon dioxide surface; the sol-gel method and the surface imprinting technology are adopted, so as to form a protein imprint layer on the silicon dioxide surface; after the elution of a template, the quantum dot-based protein core-shell imprinted microspheres are obtained. According to the invention, after the formation of the protein imprint layer, due to the existence of the surface adsorption action, the fluorescence of the quantum dots is weakened for quenching; after the elution of the template molecules, the fluorescence of the quantum dots is restored and strengthened; therefore, high-efficient recognition and high- sensitive fluorescence detection can be conducted on the template molecules phycocyanin. The preparation method for the quantum dot-based protein core-shell imprinted microspheres has the advantages of speediness, high selectivity, high sensitivity, easy operation, low cost and the like; related study on molecule imprint / protein is enriched.
; 张忠; 李金花
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