The invention belongs to the technical fields of materials science and engineering and micro-fluidic chips and specifically relates to a preparation method of a protein blot paper chip based on quantum dots. The preparation method comprises the following steps : firstly, performing amino modification on the surface of a paper chip substrate to enable the surface to have the CdTe quantum dots with carboxyls, and then grafting the quantum dots to the surface of a paper chip; next, synthesizing a protein blot layer on the surface of the paper chip by use of a sol-gel method and a surface blotting technique, washing off template molecules, and immobilizing the chip on paper having waxed micro-flow channels, thereby obtaining the protein blot paper chip. The preparation method of the protein blot paper chip based on the quantum dots has the advantages of fastness, portability, economic efficiency, high sensitivity and the like; a new policy is provided for detecting phycocyanin and the relevant study of the paper chip is enriched.
; 张忠; 李博伟
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