The invention relates to the field of environmental monitoring by utilizing biological materials and in particular relates to a method for detecting trace bivalent copper ions by utilizing a bio-element. The method comprises the following steps : enabling a buffer solution and a fluorescent iron carrier solution P to react in a water environment at room temperature for 5-10 minutes, thereby obtaining a reaction solution A for later use; enabling the buffer solution and the fluorescent iron carrier solution P to react in a to-be-detected sample environment at room temperature for 5-10 minutes, thereby obtaining a reaction solution B for later use; collecting different emission spectrums of the reaction solution A and the reaction solution B in the wavelength range of 420-600nm, calculating the fluorescence quenching rate alpha by virtue of the spectrums, and calculating the concentration of the bivalent copper ions in the sample according to a standard curve, wherein the buffer solution refers to 1-80mM N-2-hydroxyethylpiperazine-N-ethane-sulphonicacid buffer solution, and the pH value is 7-8. The method disclosed by the invention is simple and convenient to operate, short in reaction time and low in cost.
; 殷堃; 柴百惠; 张卫卫
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