The invention relates to detection of monoamine oxidase, and specifically relates to an organic compound based on cyanine and an application of the organic compound. The organic compound is shown in astructural formula I, and the compound is used as a fluorescent probe for ratio detection of monoamine oxidase A (MAO-A) on mitochondrial outer membranes. According to the compound used as the fluorescent probe for the ratio detection of the MAO-A, in the presence of the MAO-A, a response group is used as a substrate of the MAO-A; and under the action of an FAD coenzyme, methylamino is oxidized to form an aldehyde group under catalysis of the MAO-A, beta-elimination is carried out on a propionaldehyde part, and CO2 is released spontaneously, so that a fluorescent group is released. The organic compound disclosed by the invention is used for the ratio detection of the MAO-A, and in the presence of the MAO-A, obvious displacement of the corresponding fluorescence wavelength occurs, so thatthe organic compound can be used for the detection of the MAO-A, the interference of external detection conditions can be greatly reduced, and the detection precision is improved. According to the compound used as the fluorescent probe for the ratio detection of the MAO-A, when the MAO-A exists, the ultraviolet absorption changes obviously, and an ultraviolet spectrophotometer and naked eyes can be simultaneously used for detection.
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