The invention relates to the technical field of electrochemical analysis, biological immunity and micro-fluidic chips, in particular to a rotary type electrochemical immunoassay paper chip analysis device and a detection method thereof. The device comprises an auxiliary disc, a reagent disc and a washing disc which are rotationally connected, wherein the auxiliary disc, the reagent disc and the washing disc can independently rotate; a respective fluid passage is formed in each of the auxiliary disc, the reagent disc and the washing disc; holes are formed in each fluid passage; the fluid is controlled to flow in each hole when the holes formed in the auxiliary disc, the reagent disc and the washing disc and are rotated to the mutually communicated holes. Each layer of paper chips in the device can independently rotate around the center, so that a rotating valve is ingeniously integrated onto the paper chip device for controlling the flowing of fluid on the paper chip. The preparation ofthe device in the invention is simple and fast; the operation is convenient; a novel device is provided for the fast and convenient medical instant diagnosis.
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