The invention belongs to the field of microbial technology application, and particularly relates to a method for improving the hydrogen yield of hydrogenogens. The hydrogenogens strains are inoculatedinto a culture medium containing 2.5 to 20 mmol/L ferriferous oxides; through dark fermentation culture, the great-quantity hydrogen generation of the hydrogenogens strains can be realized. Through the addition of ferrihydrite and magnetite, the growth of hydrogen production clostridium C.pasteurianum is promoted; the C.pasteurianum substrate conversion efficiency is accelerated; the yield of metabolite products of hydrogen gas, acetic acid and butyric acid is improved. The method provided by the invention has the advantages that the hydrogen yield of the hydrogen production clostridium is greatly improved; the theoretical support is provided for the large-scale production of microbial dark fermentation hydrogen production.
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