本发明涉及基因工程技术领域，具体涉及一种斜生栅藻叶绿体同源重组空载体及其应用。载体包括上下游同源臂，以及同源臂间设启动子和终止子，启动子和终止子之间插入与至少一个外源基因构成的多顺反子结构的SEQ ID NO : 6所示的碱基序列；其中，上游同源臂含SEQ ID NO : 1所示碱基序列，下游同源臂含SEQ ID NO : 2所示碱基序列。采用本发明的斜生栅藻叶绿体同源重组空载体可实现多个外源基因在叶绿体中稳定表达。
其他摘要
The invention relates to the technical field of genetic engineering, in particular to a scenedesmus obliquus chloroplast homologous recombination empty vector and application thereof. The scenedesmusobliquus chloroplast homologous recombination empty vector includes an upstream homologous arm, a downstream homologous arm, a promoter and a terminator, the promoter and the terminator are arranged between the upstream homologous arm and the downstream homologous arm, and a base sequence is inserted between the promoter and the terminator, wherein the base sequence is shown in SEQ ID NO : 6, and the base sequence and at least one exogenous gene form a polycistron structure, the upstream homologous arm contains a base sequence shown in SEQ ID NO : 1, and the downstream homologous arm contains a base sequence shown in SEQ ID NO : 2. By adopting the scenedesmus obliquus chloroplast homologous recombination empty vector, several exogenous genes can be stably expressed in chloroplast.
; 王康; 任家利; 秦松
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