The enriching and screening process of antimony reducing bacteria includes the following steps : deoxidizing marine sediment in enriched culture medium containing 0.01-100 mm sodium lactate and 0.01-50 mm KSb (OH) 6, culturing in dark and constant temperature condition, and culturing the enriched culture medium containing reducing agent including at least one of NaSO4, Na2S2O3, FeSO4, ascorbic acid and ferric citrate; Replacing enriched culture medium every 1-10 days, culturing for 5-10 rounds, aerobic screening with enriched culture medium to obtain facultative anaerobic bacteria with antimony reducing capacity; Rescreening was carried out using a selection medium containing 18 mm sodium lactate and 1 mm sb (v) and the resulting discoloration of the selection medium was antimony reducing bacteria. According to the enrichment and screening method of the antimony-reducing bacteria, reducing agents such as iron-containing compounds or sulfur-containing compounds are added into the enrichment culture medium, so that the abundance of target functional microorganisms can be greatly improved, and the antimony reduction efficiency and the screening success rate of the microorganisms can be improved.
修改评论