本发明公开了一株可产褐藻胶裂解酶的勒克氏菌及其应用，其中，该勒克氏菌分离自从黄海处采摘的腐烂的马尾藻，其生长周期短、可产褐藻胶裂解酶，并具所产的褐藻胶裂解酶具有较高的酶活，该勒克氏菌已于2019年4月28日进行了菌种保藏，保藏地点为：中国典型培养物保藏中心，保藏编号为：CCTCC M 2019314。本发明的有益之处在于：(1)筛选得到的菌株AlgM可产高活性褐藻胶裂解酶，降解褐藻胶的能力增强，褐藻寡糖的产量提高；(2)筛选得到的菌株AlgM生长周期较短且能同时以海藻酸钠、葡萄糖、蛋白胨为碳源生长，能够高效产酶；(3)种子培养基和发酵培养基都不含无机盐，简化了褐藻寡糖的分离纯化过程。
其他摘要
The invention discloses Leclercia sp. capable of producing alginate lyase and application of the Leclercia sp.. The Leclercia sp. is separated from decayed Sargassum which is picked from the Yellow Sea, has a short growth cycle, can be applied to production of alginate lyase, and the produced alginate lyase has high enzyme activity. Culture preservation of the Leclercia sp. is performed on April 28, 2019 in China Typical Culture Collection Center, and the deposit number is CCTCC M 2019314. The Leclercia sp. has the following advantages that (1) high-activity alginate lyase can be produced by the screened strain AlgM, the ability of alginate degradation is enhanced, and the yield of alginate oligosaccharide is increased; (2) the growth period of the screened strain AlgM is short, and meanwhile growth is performed through adoption of sodium alginate, glucose and peptone as carbon sources, and enzymes can be produced efficiently; and (3) no inorganic salt is contained in both a seed culture medium and a fermentation culture medium, and therefore the process of separation and purification of alginate oligosaccharide is simplified.
; 秦松
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