Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection

doi:10.1021/acs.analchem.3c01348

	Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection
	Wu, Yixuan 1; Yu, Qian 1; Joung, Younju 1; Jeon, Chang Su 2; Lee, Seunghyun 3; Pyun, Sung Hyun 2; Joo, Sang-Woo 4; Chen, Lingxin5,6 ; Choo, Jaebum 1
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
	2023-08-18
页码	9
DOI	10.1021/acs.analchem.3c01348
通讯作者	Pyun, Sung Hyun(ceo@speclipse.com) ; Joo, Sang-Woo(sjoo@ssu.ac.kr) ; Chen, Lingxin(lxchen@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr)
英文摘要	Wereport the development of a reproducible and highly sensitivesurface-enhanced Raman scattering (SERS) substrate using a butanol-inducedself-assembly of gold nanoparticles (AuNPs) and its application asa rapid diagnostic platform for severe acute respiratory syndromecoronavirus 2 (SARS-CoV-2). The butanol-induced self-assembly processwas used to generate a uniform assembly of AuNPs, with multiple hotspots,to achieve high reproducibility. When an aqueous droplet containingAuNPs and target DNAs was dropped onto a butanol droplet, butanol-induceddehydration occurred, enriching the target DNAs around the AuNPs andincreasing the loading density of the DNAs on the AuNP surface. TheSERS substrate was evaluated by using Raman spectroscopy, which showedstrong electromagnetic enhancement of the Raman signals. The substratewas then tested for the detection of SARS-CoV-2 using SERS, and avery low limit of detection (LoD) of 3.1 x 10(-15) M was obtained. This provides sufficient sensitivity for the SARS-CoV-2screening assay, and the diagnostic time is significantly reducedas no thermocycling steps are required. This study demonstrates amethod for the butanol-induced self-assembly of AuNPs and its applicationas a highly sensitive and reproducible SERS substrate for the rapiddetection of SARS-CoV-2. The results suggest the potential of thisapproach for developing rapid diagnostic platforms for other biomoleculesand infectious diseases.
资助机构	National Research Foundation of Korea ; National Natural Science Foundation of China
收录类别	SCI
语种	英语
关键词[WOS]	NANOSTRUCTURES ; AGGREGATION ; IMMUNOASSAY ; SUPPRESSION ; PARTICLES
研究领域[WOS]	Chemistry
WOS记录号	WOS:001050803100001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/33448
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Pyun, Sung Hyun; Joo, Sang-Woo; Chen, Lingxin; Choo, Jaebum
作者单位	1.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea 2.Speclipse Inc, R&D Ctr, Seongnam 13461, South Korea 3.Hanyang Univ ERICA, Dept Chem & Mol Engn, Ansan 15588, South Korea 4.Soongsil Univ, Dept Chem, Seoul 06978, South Korea 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 6.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Wu, Yixuan,Yu, Qian,Joung, Younju,et al. Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection[J]. ANALYTICAL CHEMISTRY,2023:9.
APA	Wu, Yixuan.,Yu, Qian.,Joung, Younju.,Jeon, Chang Su.,Lee, Seunghyun.,...&Choo, Jaebum.(2023).Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection.ANALYTICAL CHEMISTRY,9.
MLA	Wu, Yixuan,et al."Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection".ANALYTICAL CHEMISTRY (2023):9.