How iron-bearing minerals affect the biological reduction of Sb(V): A newly discovered function of nitrate reductase

doi:10.1016/j.scitotenv.2023.167001

	How iron-bearing minerals affect the biological reduction of Sb(V): A newly discovered function of nitrate reductase
	Zhang, Haikun; Sun, Yanyu; Cheng, Manman; Sui, Xiaori; Huang, Yanyan; Hu, Xiaoke
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
	2023-12-15
卷号	904 页码:11
关键词	Antimony Iron-bearing minerals Biological reduction Nitrate reductase Inhibition
DOI	10.1016/j.scitotenv.2023.167001
通讯作者	Zhang, Haikun(hkzhang@yic.ac.cn) ; Hu, Xiaoke(xkhu@yic.ac.cn)
英文摘要	As a toxic element of global concern, the elevated concentration of antimony (Sb) in the environment has attracted increasing attention. Microorganisms have been reported as important driving forces for Sb transformation. Iron (Fe) is the most important metal associated element of Sb, however, how Fe-bearing minerals affect the biological transformation of Sb is still unclear. In this study, the effects of Fe-bearing minerals on biological Sb(V) reduction were investigated by employing a marine Shewanella sp. CNZ-1 (CNZ-1). Our results showed that the presence of hematite, magnetite and ferrihydrite (1 g/L) resulted in a decrease in Sb(III) concentration of similar to 19-31 % compared to the Fe(III)-minerals free system. The calculated Sb(V) reduction rates are 0.0256 (R-2 0.71), 0.0389 (R-2 0.87), 0.0299 (R-2 0.96) and 0.0428 (R-2 0.95) h(-1) in the hematite-, magnetite-, ferrihydrite-supplemented and Fe(III)-minerals free systems, respectively. The cube-shaped Sb2O3 was characterized as a reductive product by using XRD, XPS, FTIR, TG and SEM approaches. Differential proteomic analysis showed that flagellar protein, cytochrome c, electron transfer flavoprotein, nitrate reductase and polysulfide reductase (up-regulation >1.5-fold, p value <0.05) were supposed to be included in the electron transport pathway of Sb(V) reduction by strain CNZ-1, and the key role of nitrate reductases was further highlighted during this reaction process based on the RT-qPCR and confirmatory experiments. Overall, these findings are beneficial to understand the environmental fate of Sb in the presence of Fe-bearing minerals and provide guidance in developing the bacteria/enzyme-mediated control strategy for Sb pollution.
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; CSC Scholarship
收录类别	SCI
语种	英语
关键词[WOS]	ANTIMONY ; FERRIHYDRITE ; SB(III) ; PHOTOOXIDATION ; ADSORPTION ; BACTERIUM ; SEDIMENTS ; GROWTH ; WATERS ; SOILS
研究领域[WOS]	Environmental Sciences & Ecology
WOS记录号	WOS:001079622100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32804
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Zhang, Haikun; Hu, Xiaoke
作者单位	Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Haikun,Sun, Yanyu,Cheng, Manman,et al. How iron-bearing minerals affect the biological reduction of Sb(V): A newly discovered function of nitrate reductase[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,904:11.
APA	Zhang, Haikun,Sun, Yanyu,Cheng, Manman,Sui, Xiaori,Huang, Yanyan,&Hu, Xiaoke.(2023).How iron-bearing minerals affect the biological reduction of Sb(V): A newly discovered function of nitrate reductase.SCIENCE OF THE TOTAL ENVIRONMENT,904,11.
MLA	Zhang, Haikun,et al."How iron-bearing minerals affect the biological reduction of Sb(V): A newly discovered function of nitrate reductase".SCIENCE OF THE TOTAL ENVIRONMENT 904(2023):11.