Fungi and cercozoa regulate methane-associated prokaryotes in wetland methane emissions

doi:10.3389/fmicb.2022.1076610

	Fungi and cercozoa regulate methane-associated prokaryotes in wetland methane emissions
	Wang, Linlin 1; Zhao, Mingliang 2; Du, Xiongfeng 3,4; Feng, Kai 3,4; Gu, Songsong 3; Zhou, Yuqi 5,6; Yang, Xingsheng 3,4; Zhang, Zhaojing 1; Wang, Yingcheng 7; Zhang, Zheng 1; Zhang, Qi 1; Xie, Baohua8,9 ; Han, Guangxuan8,9 ; Deng, Ye 1,3,4
发表期刊	FRONTIERS IN MICROBIOLOGY
	2023-01-06
卷号	13 页码:15
关键词	wetland inundation level CH4 flux sediment microbiota interdomain network
DOI	10.3389/fmicb.2022.1076610
通讯作者	Deng, Ye(yedeng@rcees.ac.cn)
英文摘要	Wetlands are natural sources of methane (CH4) emissions, providing the largest contribution to the atmospheric CH4 pool. Changes in the ecohydrological environment of coastal salt marshes, especially the surface inundation level, cause instability in the CH4 emission levels of coastal ecosystems. Although soil methane-associated microorganisms play key roles in both CH4 generation and metabolism, how other microorganisms regulate methane emission and their responses to inundation has not been investigated. Here, we studied the responses of prokaryotic, fungal and cercozoan communities following 5 years of inundation treatments in a wetland experimental site, and molecular ecological networks analysis (MENs) was constructed to characterize the interdomain relationship. The result showed that the degree of inundation significantly altered the CH4 emissions, and the abundance of the pmoA gene for methanotrophs shifted more significantly than the mcrA gene for methanogens, and they both showed significant positive correlations to methane flux. Additionally, we found inundation significantly altered the diversity of the prokaryotic and fungal communities, as well as the composition of key species in interactions within prokaryotic, fungal, and cercozoan communities. Mantel tests indicated that the structure of the three communities showed significant correlations to methane emissions (p < 0.05), suggesting that all three microbial communities directly or indirectly contributed to the methane emissions of this ecosystem. Correspondingly, the interdomain networks among microbial communities revealed that methane-associated prokaryotic and cercozoan OTUs were all keystone taxa. Methane-associated OTUs were more likely to interact in pairs and correlated negatively with the fungal and cercozoan communities. In addition, the modules significantly positively correlated with methane flux were affected by environmental stress (i.e., pH) and soil nutrients (i.e., total nitrogen, total phosphorus and organic matter), suggesting that these factors tend to positively regulate methane flux by regulating microbial relationships under inundation. Our findings demonstrated that the inundation altered microbial communities in coastal wetlands, and the fungal and cercozoan communities played vital roles in regulating methane emission through microbial interactions with the methane-associated community.
收录类别	SCI
语种	英语
关键词[WOS]	RIBOSOMAL-RNA SEQUENCES ; 3 GORGES RESERVOIR ; COOCCURRENCE PATTERNS ; MICROBIAL COMMUNITIES ; SOIL ; DIVERSITY ; COMPETITION ; SEDIMENTS ; ZONE ; FEN
研究领域[WOS]	Microbiology
WOS记录号	WOS:000913695300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32580
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室支撑部门_中国科学院黄河三角洲滨海湿地试验站
通讯作者	Deng, Ye
作者单位	1.Shandong Univ, Inst Marine Sci & Technol, Qingdao, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, CAS Key Lab Environm Biotechnol, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 5.Zhejiang Univ, Inst Soil & Water Resources & Environm Sci, Coll Environm & Resource Sci, Hangzhou, Peoples R China 6.Zhejiang Univ, Zhejiang Prov Key Lab Agr Resources & Environm, Hangzhou, Peoples R China 7.Qinghai Univ, Collage Agr & Anim Husb, Xining, Peoples R China 8.Chinese Acad Sci, Yellow River Delta Field Observat & Res Stn Coasta, Yantai, Peoples R China 9.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remediat, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Linlin,Zhao, Mingliang,Du, Xiongfeng,et al. Fungi and cercozoa regulate methane-associated prokaryotes in wetland methane emissions[J]. FRONTIERS IN MICROBIOLOGY,2023,13:15.
APA	Wang, Linlin.,Zhao, Mingliang.,Du, Xiongfeng.,Feng, Kai.,Gu, Songsong.,...&Deng, Ye.(2023).Fungi and cercozoa regulate methane-associated prokaryotes in wetland methane emissions.FRONTIERS IN MICROBIOLOGY,13,15.
MLA	Wang, Linlin,et al."Fungi and cercozoa regulate methane-associated prokaryotes in wetland methane emissions".FRONTIERS IN MICROBIOLOGY 13(2023):15.