Aquaculture drastically increases methane production by favoring acetoclastic rather than hydrogenotrophic methanogenesis in shrimp pond sediments

doi:10.1016/j.aquaculture.2022.738999

	Aquaculture drastically increases methane production by favoring acetoclastic rather than hydrogenotrophic methanogenesis in shrimp pond sediments
	Tan, Ji 1,2,3,4; Lichtfouse, Eric 5; Luo, Min 2,4,7; Liu, Yuxiu 1,2,3; Tan, Fengfeng 1,2,3; Zhang, Changwei 1,2,4; Chen, Xin 1,2,4; Huang, Jiafang 1,2,3; Xiao, Leilei6
发表期刊	AQUACULTURE
ISSN	0044-8486
	2023-01-30
卷号	563 页码:14
关键词	Methane Coastal aquaculture pond Isotopic fractionation factors Acetoclastic methanogenesis Hydrogenotrophic methanogenesis Methanogenic community structure
DOI	10.1016/j.aquaculture.2022.738999
通讯作者	Luo, Min(luomin@fzu.edu.cn) ; Xiao, Leilei(llxiao@yic.ac.cn)
英文摘要	Emissions of methane (CH4), a major greenhouse gas, should be cut by at least 30% by 2030 according to the last Conference of the Parties, CoP26. Aquaculture pond is a major CH4 emitter, yet the microbial mechanisms ruling methanogenesis by degradation of organic matter in sediments remain unclear. In particular, the respective roles of hydrogenotrophic and acetoclastic methanogenesis, and the impact of aquaculture farming practices are unknown. We studied methanogenesis in the surface sediments from a freshwater and an oligohaline pond before, during, and after shrimp farming. Hydrogenotrophic and acetoclastic contributions were distinguished by acetoclastic inhibition with methylfluoride (CH3F), and by C-13-analysis of CO2 and CH4. We also monitored the methanogenic community structure, dissolved organic carbon (DOC) levels, carbon to nitrogen (C/N) ratios, and humification indices derived from Fourier transform infrared spectroscopy. The results reveal that aquaculture farming practices increased methanogenesis rates, and these increases were explained by higher levels of DOC and lower C/N ratios during farming. Of the total methane produced, 51%-78% was by hydrogenotrophic methanogenesis. However, the total methane contribution from acetoclastic methanogenesis increased from approximately 22% before farming to approximately 45% during and after farming, with a decreasing isotope fractionation factor alpha c and an increasing relative abundance of Methanosaeta acetoclastic methanogen. All hu-mification indices decreased during and after farming compared to before farming due to the input of polysaccharide-rich aquafeed. The close relationship between the humification indices and methanogenesis pathways indicates that the changes in sediment substrate quality drove the variation in the methanogenesis pathways. Increases in salinity decreased the methanogenesis rates but did not change the methanogenesis pathways. Overall, our findings reveal that aquaculture farming practices increase methanogenesis rates and favor acetoclastic over hydrogenotrophic methanogenesis, and that adjusting shrimp diets, increasing salinity, and removing residual aquafeed could reduce methanogenesis.
资助机构	National Science Foundation of China ; Natural Science Foundation of Fujian Province ; Science and Technology Projects of the Forest Bureau of Fujian Province ; Youth Innovation Promotion Association, CAS
收录类别	SCI
语种	英语
关键词[WOS]	MICROBIAL COMMUNITY STRUCTURE ; STABLE-ISOTOPE FRACTIONATION ; DISSOLVED ORGANIC-CARBON ; GREENHOUSE-GAS EMISSIONS ; PEARL RIVER ESTUARY ; GEN. NOV. ; SALINITY GRADIENT ; PATHWAYS ; MATTER ; LAKE
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000883062000007
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32396
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
通讯作者	Luo, Min; Xiao, Leilei
作者单位	1.Fujian Normal Univ, Key Lab Humid Subtrop Ecogeog Proc, Minist Educ, Fuzhou 350007, Peoples R China 2.Fuzhou Univ, Res Ctr Geog & Ecol Environm, Fuzhou 350116, Peoples R China 3.Fujian Normal Univ, Coll Geog Sci, Fuzhou 35008, Peoples R China 4.Fuzhou Univ, Coll Environm & Safety Engn, Fuzhou 350116, Peoples R China 5.Aix Marseille Univ, CNRS, IRD, INRAE,CEREGE, Ave Louis Philibert, F-13100 Aixen Provence, France 6.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 7.Fuzhou Univ, Coll Environm & Safety Engn, Wulongjiang North Ave St 2, Fuzhou 350116, Minhou County, Peoples R China
推荐引用方式 GB/T 7714	Tan, Ji,Lichtfouse, Eric,Luo, Min,et al. Aquaculture drastically increases methane production by favoring acetoclastic rather than hydrogenotrophic methanogenesis in shrimp pond sediments[J]. AQUACULTURE,2023,563:14.
APA	Tan, Ji.,Lichtfouse, Eric.,Luo, Min.,Liu, Yuxiu.,Tan, Fengfeng.,...&Xiao, Leilei.(2023).Aquaculture drastically increases methane production by favoring acetoclastic rather than hydrogenotrophic methanogenesis in shrimp pond sediments.AQUACULTURE,563,14.
MLA	Tan, Ji,et al."Aquaculture drastically increases methane production by favoring acetoclastic rather than hydrogenotrophic methanogenesis in shrimp pond sediments".AQUACULTURE 563(2023):14.