Highly Sensitive and Reliable Internal-Standard Surface-Enhanced Raman Scattering Microneedles for Determination of Bacterial Metabolites as Infection Biomarkers in Skin Interstitial Fluid

doi:10.1021/acs.analchem.2c03208

	Highly Sensitive and Reliable Internal-Standard Surface-Enhanced Raman Scattering Microneedles for Determination of Bacterial Metabolites as Infection Biomarkers in Skin Interstitial Fluid
	Mei, Rongchao 1,2,3; Wang, Yunqing2 ; Shi, Shang 2; Zhao, Xizhen 2; Zhang, Zhiyang2 ; Wang, Xiaoyan 3; Shen, Dazhong 1; Kang, Qi 1; Chen, Lingxin2,4
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
	2022-11-10
页码	10
DOI	10.1021/acs.analchem.2c03208
通讯作者	Wang, Yunqing(yqwang@yic.ac.cn) ; Kang, Qi() ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	Microneedles (MNs) are currently one of the most promising tools for skin interstitial fluid (ISF)-based biosensing, while it is still a challenge to expand the detectable biomarkers in ISF due to limited MNs types and detection techniques. Herein, highly sensitive internal-standard surface-enhanced Raman scattering microneedles (IS-SERS-MNs) were developed, which enabled the reliable detection of bacterial metabolites in ISF as new detectable biomarkers for infection diagnosis. The developed IS-SERS-MNs can not only directly detect pyocyanin (a representative bacterial metabolite) present in mouse dermal ISF but also indirectly detect pyocyanin in the hypodermis via its diffusion into the dermis, revealing a new possible pathway for the source of biomarkers in dermal ISF. Moreover, the SERS signal of pyocyanin was also clearly detected at real mouse wounds, indicating that the developed IS-SERS-MNs have great potential in minimally invasive and painless diagnosis of bacterial infection via a new ISF route. This work not only develops IS-SERS-MNs as a powerful tool for expanding the application of SERS-based MNs but also provides a new chance for ISF-related infection diagnosis.
资助机构	National Natural Science Foundation of China ; Science and Technology Development Plan of Shandong Province of China ; Youth Innovation Promotion Association CAS ; Instrument Developing Project of the Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	NANOPARTICLES
研究领域[WOS]	Chemistry
WOS记录号	WOS:000885666900001
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32371
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Wang, Yunqing; Kang, Qi; Chen, Lingxin
作者单位	1.Shandong Normal Univ, Coll Chem, Key Lab Mol & NanoProbes, Chem Engn & Mat Sci,Minist Educ, Jinan 250014, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 4.Qingdao Univ Technol, Sch Environm & Municipal Engn, Qingdao 266033, Peoples R China
推荐引用方式 GB/T 7714	Mei, Rongchao,Wang, Yunqing,Shi, Shang,et al. Highly Sensitive and Reliable Internal-Standard Surface-Enhanced Raman Scattering Microneedles for Determination of Bacterial Metabolites as Infection Biomarkers in Skin Interstitial Fluid[J]. ANALYTICAL CHEMISTRY,2022:10.
APA	Mei, Rongchao.,Wang, Yunqing.,Shi, Shang.,Zhao, Xizhen.,Zhang, Zhiyang.,...&Chen, Lingxin.(2022).Highly Sensitive and Reliable Internal-Standard Surface-Enhanced Raman Scattering Microneedles for Determination of Bacterial Metabolites as Infection Biomarkers in Skin Interstitial Fluid.ANALYTICAL CHEMISTRY,10.
MLA	Mei, Rongchao,et al."Highly Sensitive and Reliable Internal-Standard Surface-Enhanced Raman Scattering Microneedles for Determination of Bacterial Metabolites as Infection Biomarkers in Skin Interstitial Fluid".ANALYTICAL CHEMISTRY (2022):10.