Systematic Analysis of Lysine Acetylation Reveals Diverse Functions in Azorhizobium caulinodans Strain ORS571

doi:10.1128/spectrum.03539-22

	Systematic Analysis of Lysine Acetylation Reveals Diverse Functions in Azorhizobium caulinodans Strain ORS571
	Liu, Yanan 1,3; Liu, Xiaolin 1; Dong, Xiaoyan 1; Yin, Zhiqiu 2; Xie, Zhihong2 ; Luo, Yongming4
发表期刊	MICROBIOLOGY SPECTRUM
ISSN	2165-0497
	2022-12-08
页码	18
关键词	lysine acetylation acetylome Azorhizobium caulinodans ORS571 deacetylase chemotaxis
DOI	10.1128/spectrum.03539-22
通讯作者	Xie, Zhihong(zhihongxie211@163.com)
英文摘要	Protein acetylation can quickly modify the physiology of bacteria to respond to changes in environmental or nutritional conditions, but little information on these modifications is available in rhizobia. In this study, we report the lysine acetylome of Azorhizobium caulinodans strain ORS571, a model rhizobium isolated from stem nodules of the tropical legume Sesbania rostrata that is capable of fixing nitrogen in the free-living state and during symbiosis. Antibody enrichment and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis were used to characterize the acetylome. There are 2,302 acetylation sites from 982 proteins, accounting for 20.8% of the total proteins. Analysis of the acetylated motifs showed the preferences for the amino acid residues around acetylated lysines. The response regulator CheY1, previously characterized to be involved in chemotaxis in strain ORS571, was identified as an acetylated protein, and a mutation of the acetylated site of CheY1 significantly impaired the strain's motility. In addition, a Zn+-dependent deacetylase (AZC_0414) was characterized, and the construction of a deletion mutant strain showed that it played a role in chemotaxis. Our study provides the first global analysis of lysine acetylation in ORS571, suggesting that acetylation plays a role in various physiological processes. In addition, we demonstrate its involvement in the chemotaxis process. The acetylome of ORS571 provides insights to investigate the regulation mechanism of rhizobial physiology.IMPORTANCE Acetylation is an important modification that regulates protein function and has been found to regulate physiological processes in various bacteria. The physiology of rhizobium A. caulinodans ORS571 is regulated by multiple mechanisms both when free living and in symbiosis with the host; however, the regulatory role of acetylation is not yet known. Here, we took an acetylome-wide approach to identify acetylated proteins in A. caulinodans ORS571 and performed clustering analyses. Acetylation of chemotaxis proteins was preliminarily investigated, and the upstream acetylation-regulating enzyme involved in chemotaxis was characterized. These findings provide new insights to explore the physiological mechanisms of rhizobia. Acetylation is an important modification that regulates protein function and has been found to regulate physiological processes in various bacteria. The physiology of rhizobium A. caulinodans ORS571 is regulated by multiple mechanisms both when free living and in symbiosis with the host; however, the regulatory role of acetylation is not yet known.
资助机构	National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key Research and Development Program
收录类别	SCI
语种	英语
关键词[WOS]	ACETYLOME ANALYSIS REVEALS ; GRAM-NEGATIVE BACTERIA ; RESPONSE REGULATOR ; COA SYNTHETASE ; HISTONE DEACETYLASES ; DI-GMP ; CHEMOTAXIS ; PROTEINS ; BINDING ; CHEY
研究领域[WOS]	Microbiology
WOS记录号	WOS:000893843100001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31886
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Xie, Zhihong
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China 2.Shandong Agr Univ, Coll Resources & Environm, Natl Engn Res Ctr Efficient Utilizat Soil & Fertil, Tai An, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Soil Sci, CAS Key Lab Soil Environm & Pollut Remediat, Nanjing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yanan,Liu, Xiaolin,Dong, Xiaoyan,et al. Systematic Analysis of Lysine Acetylation Reveals Diverse Functions in Azorhizobium caulinodans Strain ORS571[J]. MICROBIOLOGY SPECTRUM,2022:18.
APA	Liu, Yanan,Liu, Xiaolin,Dong, Xiaoyan,Yin, Zhiqiu,Xie, Zhihong,&Luo, Yongming.(2022).Systematic Analysis of Lysine Acetylation Reveals Diverse Functions in Azorhizobium caulinodans Strain ORS571.MICROBIOLOGY SPECTRUM,18.
MLA	Liu, Yanan,et al."Systematic Analysis of Lysine Acetylation Reveals Diverse Functions in Azorhizobium caulinodans Strain ORS571".MICROBIOLOGY SPECTRUM (2022):18.