Bradyrhizobium aeschynomenes sp. nov., a root and stem nodule microsymbiont of Aeschynomene indica

doi:10.1016/j.syapm.2022.126337

	Bradyrhizobium aeschynomenes sp. nov., a root and stem nodule microsymbiont of Aeschynomene indica
	Sun, Li 1; Zhang, Zhenpeng 2; Dong, Xiaoyan 2; Tang, Zhihong 3; Ju, Bao 3; Du, Zongjun 4; Wang, Entao 5; Xie, Zhihong1
发表期刊	SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN	0723-2020
	2022-07-01
卷号	45 期号:4 页码:13
关键词	Nodules Nod-independent Taxonomy Bradyrhizobium aeschynomenes sp nov
DOI	10.1016/j.syapm.2022.126337
通讯作者	Xie, Zhihong(zhihongxie211@163.com)
英文摘要	Aeschynomene indica has a distinctive symbiosis with Bradyrhizobium in which nodulation is Nod factorindependent. In this study, we characterised three Gram-negative and rod-shaped strains (83002T, 81013 and 83012) isolated from root nodules of Aeschynomene indica in Shandong Peninsula. The major cellular fatty acids of isolates were C16:0, C18:0, C18:1 x7c 11-methyl, summed feature 3 and summed feature 8. The major polar lipids were phosphatidylethanolamine (PE), aminolipids (AL) and phosphatidylcholine (PC). Phylogenetic analysis based on the 16S rRNA locus showed that they belonged to the Bradyrhizobium genus, and shared the highest similarity to the type strains Bradyrhizobium oligotrophicum S58T and Bradyrhizobium denitrificans LMG 8443T. As expected, analysis of concatenated sequences of six housekeeping genes (atpD, recA, glnII, dnaK, gyrB, and rpoB) and nifH gene proposed that these three strains formed a distinct clade within the genus Bradyrhizobium. The highest average nucleotide identity and DNA-DNA hybridization values of the three strains in comparison to the closest Bradyrhizobium species were 87.5% and 65.3%, respectively, which are far below the threshold of species delineation, and thus confirmed the three strains as a new species. The genome size of strain 83002T is 7.52 Mbp, and the DNA G+C content is 65.42 mol%. Strain 83002T (=KCTC 82266T=MCCC 1K04775T) was chosen as the type strain of the new species, for which the name Bradyrhizobium aeschynomenes sp. nov. was proposed.
资助机构	National Natural Science Foundation of China ; NSFC-Shandong Joint Fund Key Projects ; National Key Research and Development Program
收录类别	SCI
语种	英语
关键词[WOS]	NITROGEN-FIXING SYMBIONTS ; AGROMONAS-OLIGOTROPHICA ; EVOLUTIONARY GENETICS ; GENUS BRADYRHIZOBIUM ; BACTERIUM ; RHIZOBIUM ; MESORHIZOBIUM ; DIVERSITY ; ACID ; RECLASSIFICATION
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000866228300003
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31773
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
通讯作者	Xie, Zhihong
作者单位	1.Shandong Agr Univ, Coll Resources & Environm, Natl Engn Lab Efficient Utilizat Soil & Fertilizer, Tai An 271000, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China 4.Shandong Univ Weihai, Coll Marine Sci, Weihai 264209, Peoples R China 5.Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City, Mexico
推荐引用方式 GB/T 7714	Sun, Li,Zhang, Zhenpeng,Dong, Xiaoyan,et al. Bradyrhizobium aeschynomenes sp. nov., a root and stem nodule microsymbiont of Aeschynomene indica[J]. SYSTEMATIC AND APPLIED MICROBIOLOGY,2022,45(4):13.
APA	Sun, Li.,Zhang, Zhenpeng.,Dong, Xiaoyan.,Tang, Zhihong.,Ju, Bao.,...&Xie, Zhihong.(2022).Bradyrhizobium aeschynomenes sp. nov., a root and stem nodule microsymbiont of Aeschynomene indica.SYSTEMATIC AND APPLIED MICROBIOLOGY,45(4),13.
MLA	Sun, Li,et al."Bradyrhizobium aeschynomenes sp. nov., a root and stem nodule microsymbiont of Aeschynomene indica".SYSTEMATIC AND APPLIED MICROBIOLOGY 45.4(2022):13.