The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant

doi:10.3389/fmicb.2021.744268

	The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant
	Sun, Li 1; Wang, Dandan 1; Yin, Zhiqiu 1; Zhang, Chengsheng 2; Bible, Amber 3; Xie, Zhihong1,4
发表期刊	FRONTIERS IN MICROBIOLOGY
	2021-11-19
卷号	12 页码:13
关键词	Azorhizobium caulinodans ORS571 transcriptional regulator flagella exopolysaccharide biofilm symbiosis
DOI	10.3389/fmicb.2021.744268
通讯作者	Zhang, Chengsheng(zhangchengsheng@caas.cn) ; Xie, Zhihong(zhihongxe211@163.com)
英文摘要	Bacterial signal transduction pathways are important for a variety of adaptive responses to environment, such as two-component systems (TCSs). In this paper, we reported the characterization of a transcriptional regulator in Azorhizobium caulinodans ORS571, ActR, with an N-terminal receiver domain and one C-terminal OmpR/PhoB-type DNA binding domain. Sequence analysis showed that ActR shared a high similarity with FtcR regulator of Brucella melitensis 16M known to be involved in flagellar regulation. The structural gene of this regulator was largely distributed in Alphaproteobacteria, in particular in Rhizobiales and Rhodobacterales, and was located within clusters of genes related to motility functions. Furthermore, we studied the biological function of ActR in A. caulinodans grown at the free-living state or in association with Sesbania rostrata by constructing actR gene deletion mutant. In the free-living state, the bacterial flagellum and motility ability were entirely deleted, the expression of flagellar genes was downregulated; and the exopolysaccharide production, biofilm formation, and cell flocculation decreased significantly compared with those of the wild-type strain. In the symbiotic state, Delta actR mutant strain showed weakly competitive colonization and nodulation on the host plant. These results illustrated that FtcR-like regulator in A. caulinodans is involved in flagellar biosynthesis and provide bacteria with an effective competitive nodulation for symbiosis. These findings improved our knowledge of FtcR-like transcriptional regulator in A. caulinodans.
收录类别	SCI
语种	英语
关键词[WOS]	NITROGEN-FIXATION ; ESCHERICHIA-COLI ; FLAGELLA ; SYSTEM ; LIPOPOLYSACCHARIDE ; EXOPOLYSACCHARIDES ; POLYSACCHARIDE ; AZOSPIRILLUM ; CHEMOTAXIS ; REGULATOR
研究领域[WOS]	Microbiology
WOS记录号	WOS:000726360400001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/30939
专题	中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Zhang, Chengsheng; Xie, Zhihong
作者单位	1.Shandong Agr Univ, Coll Resources & Environm, Natl Engn Lab Efficient Utilizat Soil & Fertilize, Tai An, Shandong, Peoples R China 2.Chinese Acad Agr Sci, Tobacco Res Inst, Marine Agr Res Ctr, Qingdao, Peoples R China 3.Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Sun, Li,Wang, Dandan,Yin, Zhiqiu,et al. The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant[J]. FRONTIERS IN MICROBIOLOGY,2021,12:13.
APA	Sun, Li,Wang, Dandan,Yin, Zhiqiu,Zhang, Chengsheng,Bible, Amber,&Xie, Zhihong.(2021).The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant.FRONTIERS IN MICROBIOLOGY,12,13.
MLA	Sun, Li,et al."The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant".FRONTIERS IN MICROBIOLOGY 12(2021):13.