SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates

doi:10.1016/j.bios.2021.113736

	SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates
	Wu, Yixuan 1; Dang, Hajun 1; Park, Sung-Gyu 2; Chen, Lingxin3 ; Choo, Jaebum 1
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
	2022-02-01
卷号	197 页码:8
关键词	surface-Enhanced Raman scattering SERS-PCR SARS-CoV-2 Au nanodimple substrate Nanogap
DOI	10.1016/j.bios.2021.113736
通讯作者	Chen, Lingxin(lxchen@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr)
英文摘要	The reverse transcription-polymerase chain reaction (RT-PCR) method has been adopted worldwide to diagnose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Although this method has good sensitivity and specificity, there is a need to develop a more rapid diagnostic technology, given the virus's rapid spread. However, the RT-PCR method takes a long time to diagnose SARS-CoV-2 because of the required thermocycling steps. Therefore, we developed a surface-enhanced Raman scattering (SERS)-PCR detection method using an AuNP-internalized Au nanodimple substrate (AuNDS) to shorten the diagnosis time by reducing the number of thermocycling steps needed to amplify the DNA. For the representative target markers, namely, the envelope protein (E) and RNA-dependent RNA polymerase (RdRp) genes of SARS-CoV-2, 25 RT-PCR thermocycles are required to reach a detectable threshold value, while 15 cycles are needed for magnetic bead-based SERS-PCR when the initial DNA concentration was 1.00x 10(5) copies/mu L. However, only 8 cycles are needed for the AuNDS-based SERS-PCR. The corresponding detectable target DNA concentrations were 3.36 x 10(12), 3.28 x 10(9), and 2.56 x 10(7) copies/mu L, respectively. Therefore, AuNDS-based SERS-PCR is seen as being a new molecular diagnostic platform that can shorten the time required for the thermocycling steps relative to the conventional RTPCR.
资助机构	National Research Foundation of Korea ; Ministry of Trade, Industry and Energy ; National Natural Science Foundation of China of China ; Taishan Scholar Project Special Funding
收录类别	SCI
语种	英语
研究领域[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS记录号	WOS:000719370300002
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/30918
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Chen, Lingxin; Choo, Jaebum
作者单位	1.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea 2.Korea Inst Mat Sci KIMS, Nanobio Convergence Dept, Chang Won 51508, South Korea 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Wu, Yixuan,Dang, Hajun,Park, Sung-Gyu,et al. SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates[J]. BIOSENSORS & BIOELECTRONICS,2022,197:8.
APA	Wu, Yixuan,Dang, Hajun,Park, Sung-Gyu,Chen, Lingxin,&Choo, Jaebum.(2022).SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates.BIOSENSORS & BIOELECTRONICS,197,8.
MLA	Wu, Yixuan,et al."SERS-PCR assays of SARS-CoV-2 target genes using Au nanoparticles-internalized Au nanodimple substrates".BIOSENSORS & BIOELECTRONICS 197(2022):8.