Identification, antibacterial activities and action mode of two macins from manila clam Venerupis philippinarum

doi:10.1016/j.fsi.2021.09.031

	Identification, antibacterial activities and action mode of two macins from manila clam Venerupis philippinarum
	Xue, Rui 1,3; Han, Yijing 1,4; Li, Fan 5; Chen, Lizhu 5; Yang, Dinglong1,2,3 ; Zhao, Jianmin1,2,3
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2021-11-01
卷号	118 页码:411-420
关键词	Antimicrobial peptide Macin Venerupis philippinarum Antibacterial activity
DOI	10.1016/j.fsi.2021.09.031
通讯作者	Yang, Dinglong(dlyang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn)
英文摘要	In this study, two macins were identified from clam Venerupis philippinarum (designated as VpMacin-1 and VpMacin-2). They showed 64.71% similarity with each other. The highest mRNA expression of VpMacin-1 and VpMacin-2 was detected in gills and hepatopancreas, respectively, in non-stimulated clams, and their expression could be induced significantly in hemocytes after Vibrio anguillarum infection. Silencing of VpMacin-1 and VpMacin-2 led to 22% and 49% mortality 6 days post infection. Escherichia coli cells were killed by recombinant protein rVpMacin-1 and rVpMacin-2 within 1000 and 400 min, respectively, at a concentration of 1.0 x MIC. Compared with rVpMacin-1, rVpMacin-2 not only showed higher broad-spectrum antimicrobial activities towards Vibrio strains, but possessed stronger abilities to inhibit the formation of bacterial biofilm. Both membrane integrity and electrochemical assay indicated that rVpMacins were capable of causing bacterial membrane permeabilization, especially for rVpMacin-2. Besides, rVpMacin-1 significantly induced both phagocytic (0.1 and 1.0 x MIC, p < 0.05) and chemotactic effects (0.1 x MIC, p < 0.01) of hemocytes, while there was no significant increase for rVpMacin-2. Overall, our results suggested that VpMacin-1 and VpMacin-2 play important roles in host defense against invasive pathogens.
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association of CAS
收录类别	SCI
语种	英语
关键词[WOS]	ANTIMICROBIAL PEPTIDES ; MOLECULAR CHARACTERIZATION ; CHALLENGE ; DEFENSIN ; EXPRESSION ; MUSSELS ; FAMILY ; LEECH
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000705784800005
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/30797
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Yang, Dinglong; Zhao, Jianmin
作者单位	1.Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264117, Shandong, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Xue, Rui,Han, Yijing,Li, Fan,et al. Identification, antibacterial activities and action mode of two macins from manila clam Venerupis philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2021,118:411-420.
APA	Xue, Rui,Han, Yijing,Li, Fan,Chen, Lizhu,Yang, Dinglong,&Zhao, Jianmin.(2021).Identification, antibacterial activities and action mode of two macins from manila clam Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,118,411-420.
MLA	Xue, Rui,et al."Identification, antibacterial activities and action mode of two macins from manila clam Venerupis philippinarum".FISH & SHELLFISH IMMUNOLOGY 118(2021):411-420.