Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana
Wang, Wenjing; Sheng, Yanqing
Source PublicationWORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
2021-01
Volume37Issue:1Pages:3
KeywordAmpicillin Carotenoid biosynthesis Chlorella sorokiniana Fatty acid biosynthesis Porphyrin and chlorophyll metabolism
DOI10.1007/s11274-020-02985-1
AbstractAmpicillin sodium salt (AMP) is commonly and effectively used to prevent bacterial infection in algal culture, but the response of algal strains to AMP has not been investigated. In this study, Chlorella sorokiniana was selected to evaluate the influence of AMP on algae. AMP enhanced the contents of chlorophyll and two fatty acids, myristic acid (C22:1N9) and tetracosanoic acid (C6:0), but inhibited the growth, carotenoid production, and contents of 16 fatty acids in C. sorokiniana. A global transcriptome analysis from experimental data identified 3 825 upregulated and 1 432 downregulated differentially expressed genes (DEGs) in C. sorokiniana. The upregulated DEGs, such as hemB/alaD, mmaB/pduO, cox15/ctaA, fxN, cpoX/hemF, and earS/gltX, were enriched in the porphyrin and chlorophyll metabolism pathways, whereas the downregulated DEGs, including lcyB (crtL1), crtY (lcyE, crtL2), lut1 (CYP97C1), z-isO, crtZ and crtisO (crtH), were enriched in the carotenoid biosynthesis pathway, and the downregulated DEGs, abH, fadD, fabF, acsL, fabG, and accD were enriched in the fatty acid biosynthesis pathway. Thus, the use of AMP to obtain an axenic strain revealed that AMP might affect the regulatory dynamics and the results of the metabolic process in C. sorokiniana. The data obtained in the study provide foundational information for algal purification and aseptic processing.Graphic abstract
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31600370, 41373100] ; Science and Technology Plan of Yantai City [2018ZHGY080, 2018ZHGY083] ; Regional Key Project of STS of the Chinese Academy of Sciences [KFJ-STS-QYZX-057]
Indexed BySCI
Language英语
WOS KeywordRNA-SEQ DATA ; AXENIC CULTURES ; GENE-TRANSFER ; BACTERIA ; CAROTENOIDS ; ALGAE ; ANTIBIOTICS ; CULTIVATION ; EXPRESSION ; EVOLUTION
WOS Research AreaBiotechnology & Applied Microbiology
WOS IDWOS:000606495400002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/30506
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
AffiliationChinese Acad Sci, Res Ctr Coastal Environm Engn Technol Shandong Pr, Yantai Inst Coastal Zone Res, Yantai, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Wang, Wenjing,Sheng, Yanqing. Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2021,37(1):3.
APA Wang, Wenjing,&Sheng, Yanqing.(2021).Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,37(1),3.
MLA Wang, Wenjing,et al."Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 37.1(2021):3.
Files in This Item: Download All
File Name/Size DocType Version Access License
Ampicillin used in a(3283KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Wenjing]'s Articles
[Sheng, Yanqing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Wenjing]'s Articles
[Sheng, Yanqing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Wenjing]'s Articles
[Sheng, Yanqing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Ampicillin used in aseptic processing influences the production of pigments and fatty acids in Chlorella sorokiniana.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.