| Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis | |
Gong, Fanwu1; Wei, Hua-xing2; Qi, Ji3; Ma, Huan1; Liu, Lianxin4; Weng, Jianping5; Zheng, Xucai1; Li, Qiangsheng1; Zhao, Dan1; Fang, Haopeng1; Liu, Liu1; He, Hongliang2; Ma, Cuichen1; Han, Jinglong6; Sun, Anyuan2; Wang, Baolong2; Jin, Tengchuan1; Li, Bowei3 ; Li, Bofeng1
| |
| 发表期刊 | ACS SENSORS
 |
| ISSN | 2379-3694 |
| 2021-07-23 | |
| 卷号 | 6期号:7页码:2709-2719 |
| 关键词 | SARS-Cov-2 COVID-19 diagnosis paper-based ELISA anti-RBD antibody serum separation microfluidic devices |
| DOI | 10.1021/acssensors.1c00773 |
| 通讯作者 | Jin, Tengchuan(jint@ustc.edu.cn) ; Li, Bowei(bwli@yic.ac.cn) ; Li, Bofeng(libf@ustc.edu.cn) |
| 英文摘要 | The spread of Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), resulting in a global pandemic with around four million deaths. Although there are a variety of nucleic acid-based tests for detecting SARS-CoV-2, these methods have a relatively high cost and require expensive supporting equipment. To overcome these limitations and improve the efficiency of SARS-CoV-2 diagnosis, we developed a microfluidic platform that collected serum by a pulling-force spinning top and paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) for quantitative IgA/IgM/IgG measurements in an instrument-free way. We further validated the paper-based microfluidic ELISA analysis of SARS-CoV-2 receptor-binding domain (RBD)-specific IgA/IgM/IgG antibodies from human blood samples as a good measurement with higher sensitivity compared with traditional IgM/IgG detection (99.7% vs 95.6%) for early illness onset patients. In conclusion, we provide an alternative solution for the diagnosis of SARS-CoV-2 in a portable manner by this smart integration of pulling-force spinning top and paper-based microfluidic immunoassay. |
| 资助机构 | National Natural Science Foundation of China ; COVID-19 special task grant from Chinese Academy of Science Clinical Research Hospital (Hefei) ; Shandong Provincial Natural Science Foundation Key Project ; China Postdoctoral Science Foundation |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ANTIBODIES ; CHIP |
| 研究领域[WOS] | Chemistry ; Science & Technology - Other Topics |
| WOS记录号 | WOS:000677583700023 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29598 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Jin, Tengchuan; Li, Bowei; Li, Bofeng |
| 作者单位 | 1.Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Med Oncol, Div Life Sci & Med, Hefei 230001, Peoples R China 2.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Lab Med, Hefei 230001, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Hepatobiliary Surg, Hefei 230021, Peoples R China 5.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Endocrinol & Metab, Hefei 230027, Peoples R China 6.Yantai Univ, Sch Environm & Mat Engn, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gong, Fanwu,Wei, Hua-xing,Qi, Ji,et al. Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis[J]. ACS SENSORS,2021,6(7):2709-2719. |
| APA | Gong, Fanwu.,Wei, Hua-xing.,Qi, Ji.,Ma, Huan.,Liu, Lianxin.,...&Li, Bofeng.(2021).Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis.ACS SENSORS,6(7),2709-2719. |
| MLA | Gong, Fanwu,et al."Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis".ACS SENSORS 6.7(2021):2709-2719. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Pulling-Force Spinni（5497KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论