| Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum | |
Han, Yijing1,2,3; Zhang, Qianqian1,2 ; Chen, Lizhu4; Yang, Dinglong1,2; Zhao, Jianmin1,2,5
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| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| 2021-08-01 | |
| Volume | 121Pages:10 |
| Keyword | Ruditapes philippinarum Extracellular traps Reactive oxygen species Mitochondria Antibacterial activity |
| DOI | 10.1016/j.dci.2021.104094 |
| Corresponding Author | Yang, Dinglong(dlyang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn) |
| Abstract | The formation of extracellular traps (ETs) is an important innate immune mechanism that serves to combat different invading pathogens. In this study, zymosan significantly induced the formation of ETs in the hemocytes of Ruditapes philippinarum, and this effect was accompanied by translocation of the mitochondria to the cell surface. Zymosan stimulation clearly induced an increase in intracellular ROS and MPO production and an overexpression of ROS-related genes (PI3K, AKT and HIF). In response to the ROS burst, the mitochondrial membrane potential decreased, and the mitochondrial permeability transition pore opened. Conversely, mitochondrial superoxide inhibitor (Mito-TEMPO) significantly inhibited the formation of ETs, suggesting that mitochondrial ROS were necessary for the formation of ETs. In addition, we found that zymosan-induced ETs showed antibacterial activities against gram-negative and gram-positive bacteria, such as Vibrio anguillarum, Vibrio harveyi, Escherichia coli and Micrococcus luteus. Taken together, these findings elucidated a new antibacterial approach for R. philippinarum and highlighted the role of mitochondria in the formation of zymosaninduced ETs. |
| Funding Organization | National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association, Chinese Academy of Sciences |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | VIBRIO-TAPETIS ; MANILA CLAMS ; DNA ; RELEASE ; MYELOPEROXIDASE ; IDENTIFICATION ; BACTERIA ; CARP |
| WOS Research Area | Fisheries ; Immunology ; Veterinary Sciences ; Zoology |
| WOS ID | WOS:000663315400003 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/29434 |
| Collection | 支撑部门_中国科学院牟平海岸带环境综合试验站 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| Corresponding Author | Yang, Dinglong; Zhao, Jianmin |
| Affiliation | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Shandong, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
| Recommended Citation GB/T 7714 | Han, Yijing,Zhang, Qianqian,Chen, Lizhu,et al. Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,121:10. |
| APA | Han, Yijing,Zhang, Qianqian,Chen, Lizhu,Yang, Dinglong,&Zhao, Jianmin.(2021).Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,121,10. |
| MLA | Han, Yijing,et al."Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 121(2021):10. |
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