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Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum
Han, Yijing1,2,3; Zhang, Qianqian1,2; Chen, Lizhu4; Yang, Dinglong1,2; Zhao, Jianmin1,2,5
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
2021-08-01
Volume121Pages:10
KeywordRuditapes philippinarum Extracellular traps Reactive oxygen species Mitochondria Antibacterial activity
DOI10.1016/j.dci.2021.104094
Corresponding AuthorYang, Dinglong(dlyang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn)
AbstractThe formation of extracellular traps (ETs) is an important innate immune mechanism that serves to combat different invading pathogens. In this study, zymosan significantly induced the formation of ETs in the hemocytes of Ruditapes philippinarum, and this effect was accompanied by translocation of the mitochondria to the cell surface. Zymosan stimulation clearly induced an increase in intracellular ROS and MPO production and an overexpression of ROS-related genes (PI3K, AKT and HIF). In response to the ROS burst, the mitochondrial membrane potential decreased, and the mitochondrial permeability transition pore opened. Conversely, mitochondrial superoxide inhibitor (Mito-TEMPO) significantly inhibited the formation of ETs, suggesting that mitochondrial ROS were necessary for the formation of ETs. In addition, we found that zymosan-induced ETs showed antibacterial activities against gram-negative and gram-positive bacteria, such as Vibrio anguillarum, Vibrio harveyi, Escherichia coli and Micrococcus luteus. Taken together, these findings elucidated a new antibacterial approach for R. philippinarum and highlighted the role of mitochondria in the formation of zymosaninduced ETs.
Funding OrganizationNational Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association, Chinese Academy of Sciences
Indexed BySCI
Language英语
WOS KeywordVIBRIO-TAPETIS ; MANILA CLAMS ; DNA ; RELEASE ; MYELOPEROXIDASE ; IDENTIFICATION ; BACTERIA ; CARP
WOS Research AreaFisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS IDWOS:000663315400003
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/29434
Collection支撑部门_中国科学院牟平海岸带环境综合试验站
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
Corresponding AuthorYang, Dinglong; Zhao, Jianmin
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Shandong, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Han, Yijing,Zhang, Qianqian,Chen, Lizhu,et al. Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,121:10.
APA Han, Yijing,Zhang, Qianqian,Chen, Lizhu,Yang, Dinglong,&Zhao, Jianmin.(2021).Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,121,10.
MLA Han, Yijing,et al."Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 121(2021):10.
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