| Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum | |
Han, Yijing1,2,3; Zhang, Qianqian1,2 ; Chen, Lizhu4; Yang, Dinglong1,2; Zhao, Jianmin1,2,5
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| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| 2021-08-01 | |
| 卷号 | 121页码:10 |
| 关键词 | Ruditapes philippinarum Extracellular traps Reactive oxygen species Mitochondria Antibacterial activity |
| DOI | 10.1016/j.dci.2021.104094 |
| 通讯作者 | Yang, Dinglong(dlyang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn) |
| 英文摘要 | The formation of extracellular traps (ETs) is an important innate immune mechanism that serves to combat different invading pathogens. In this study, zymosan significantly induced the formation of ETs in the hemocytes of Ruditapes philippinarum, and this effect was accompanied by translocation of the mitochondria to the cell surface. Zymosan stimulation clearly induced an increase in intracellular ROS and MPO production and an overexpression of ROS-related genes (PI3K, AKT and HIF). In response to the ROS burst, the mitochondrial membrane potential decreased, and the mitochondrial permeability transition pore opened. Conversely, mitochondrial superoxide inhibitor (Mito-TEMPO) significantly inhibited the formation of ETs, suggesting that mitochondrial ROS were necessary for the formation of ETs. In addition, we found that zymosan-induced ETs showed antibacterial activities against gram-negative and gram-positive bacteria, such as Vibrio anguillarum, Vibrio harveyi, Escherichia coli and Micrococcus luteus. Taken together, these findings elucidated a new antibacterial approach for R. philippinarum and highlighted the role of mitochondria in the formation of zymosaninduced ETs. |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association, Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | VIBRIO-TAPETIS ; MANILA CLAMS ; DNA ; RELEASE ; MYELOPEROXIDASE ; IDENTIFICATION ; BACTERIA ; CARP |
| 研究领域[WOS] | Fisheries ; Immunology ; Veterinary Sciences ; Zoology |
| WOS记录号 | WOS:000663315400003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29434 |
| 专题 | 支撑部门_中国科学院牟平海岸带环境综合试验站 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Yang, Dinglong; Zhao, Jianmin |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Shandong, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yijing,Zhang, Qianqian,Chen, Lizhu,et al. Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,121:10. |
| APA | Han, Yijing,Zhang, Qianqian,Chen, Lizhu,Yang, Dinglong,&Zhao, Jianmin.(2021).Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,121,10. |
| MLA | Han, Yijing,et al."Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 121(2021):10. |
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| Mitochondria are ess（5705KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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