| Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment | |
Zhang, Yi1; Liu, Xiao1; Wang, Lingling1; Yang, Hanjie1; Zhang, Xiaoxiao1; Zhu, Chenglong1; Wang, Wenlong1; Yan, Lijing2; Li, Bowei3
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| 发表期刊 | SCIENTIFIC REPORTS
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| ISSN | 2045-2322 |
| 2020-06-15 | |
| 卷号 | 10期号:1页码:9 |
| DOI | 10.1038/s41598-020-66456-1 |
| 通讯作者 | Zhang, Yi(zhangyijnu@jiangnan.edu.cn) |
| 英文摘要 | Lateral flow assay (LFA) is one of the most prevalent commercially available techniques for point-of-care tests due to its simplicity, celerity, low cost and robust operation. However, conventional colorimetric LFAs have inferior limits of detection (LODs) compared to sophisticated laboratory-based assays. Here, we report a simple strategy of test-zone pre-enrichment to improve the LOD of LFA by loading samples before the conjugate pad assembly. The developed method enables visual LODs of miR-210 mimic and human chorionic gonadotropin protein, to be improved by 10-100 fold compared with a conventional LFA setup without introducing any additional instrument and reagent except for phosphate running buffer, while no obvious difference occurred for Aflatoxin B1 (AFB1). It takes about 6-8min to enrich every 50 mu L of sample diluted with phosphate running buffer, therefore we can get visual results within 20min. We identified a parameter by modeling the entire process, the concentration of probe-analyte conjugate at test zone when signaling unit being loaded, to be important for the improvement of visual limit of detection. In addition, the test-zone pre-enrichment did not impair the selectivity when miR-210 mimic was adopted as target. Integrated with other optimization, amplification and modification of LFAs, the developed test-zone pre-enrichment method can be applied to further improve LOD of LFAs. |
| 资助机构 | National Natural Science Foundation of China ; Postgraduate Research & Practice Innovation Program of Jiangsu Provence |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | RECOMBINASE POLYMERASE AMPLIFICATION ; ULTRASENSITIVE DETECTION ; VISUAL DETECTION ; AFLATOXIN B-1 ; SINGLE-STEP ; SENSITIVITY ; IMMUNOASSAYS ; SIGNAL |
| 研究领域[WOS] | Science & Technology - Other Topics |
| WOS记录号 | WOS:000544936100006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/28635 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Zhang, Yi |
| 作者单位 | 1.Jiangnan Univ, State Key Lab Food Sci & Technol,Sch Food Sci & T, Int Joint Lab Food Safety,Inst Analyt Food Safety, Collaborat Innovat Ctr Food Safety & Qual Control, Wuxi 214122, Jiangsu, Peoples R China 2.Jiangnan Univ Hosp, Wuxi 214122, Jiangsu, Peoples R China 3.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yi,Liu, Xiao,Wang, Lingling,et al. Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment[J]. SCIENTIFIC REPORTS,2020,10(1):9. |
| APA | Zhang, Yi.,Liu, Xiao.,Wang, Lingling.,Yang, Hanjie.,Zhang, Xiaoxiao.,...&Li, Bowei.(2020).Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment.SCIENTIFIC REPORTS,10(1),9. |
| MLA | Zhang, Yi,et al."Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment".SCIENTIFIC REPORTS 10.1(2020):9. |
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| Improvement in Detec（4424KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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