Gene expression concerning fatty acid and amino acid metabolism inChlorella vulgariscultured with antibiotics

doi:10.1007/s00253-020-10822-6

YIC-IR > 中国科学院海岸带环境过程与生态修复重点实验室 > 海岸带环境工程技术研究与发展中心

	Gene expression concerning fatty acid and amino acid metabolism inChlorella vulgariscultured with antibiotics
	Wang, Wenjing; Sheng, Yanqing
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN	0175-7598
	2020-08-14
页码	12
关键词	Amino acid metabolism Axenic treatments Fatty acid metabolism Transcription analysis
DOI	10.1007/s00253-020-10822-6
通讯作者	Sheng, Yanqing(yqsheng@yic.ac.cn)
英文摘要	The green algaChlorella vulgarishas potential as a source of clean bioenergy with abundant metabolites and a high oil content, and antibiotics are often applied to remove bacteria from culture to obtain axenic algal strains. In this work, ceftazidime and gentamicin sulphate (GS) were added separately and in combination in the aseptic processing ofC. vulgaris, and gene expression and metabolites were evaluated. The results showed that ceftazidime and GS effectively inhibited the proliferation ofCyanobacteriaandBacteroidetes, respectively. Overall, the effects of antibiotics onC. vulgarisdiffered: GS increased the algal concentration, whereas ceftazidime alone and in combination with GS treatment decreased the specific algal growth rate. Based on comparative transcription analysis, 5917 and 5899 differentially expressed genes (DEGs) were respectively upregulated and downregulated by ceftazidime, 963 and 3921 DEGs by GS, and 4532 and 1675 DEGs by the ceftazidime and GS combination. Pathway enrichment analysis showed that the downregulated DEGs in the ceftazidime groups were enriched in the fatty acid biosynthesis pathway but that the upregulated DEGs in the GS group were enriched in the fatty acid degradation pathway. Some pathways related to amino acid metabolism were markably influenced by antibiotic treatment. The results further indicated that antibiotics affected the intracellular concentration of fatty acids and amino acids inC. vulgaris. This study provides a new viewpoint regarding the response ofC. vulgaristo antibiotics in the process of obtaining axenic algal strains.
资助机构	National Natural Science Foundation of China ; Science and Technology Plan of Yantai City ; STS of the Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	CULTIVATION ; MICROALGAE ; TOXICITY ; BIOMASS ; ABILITY ; KEGG
研究领域[WOS]	Biotechnology & Applied Microbiology
WOS记录号	WOS:000559428300001
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/28549
专题	中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Sheng, Yanqing
作者单位	Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Wenjing,Sheng, Yanqing. Gene expression concerning fatty acid and amino acid metabolism inChlorella vulgariscultured with antibiotics[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020:12.
APA	Wang, Wenjing,&Sheng, Yanqing.(2020).Gene expression concerning fatty acid and amino acid metabolism inChlorella vulgariscultured with antibiotics.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,12.
MLA	Wang, Wenjing,et al."Gene expression concerning fatty acid and amino acid metabolism inChlorella vulgariscultured with antibiotics".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020):12.

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