In Vivo Molecular Insights into Syntrophic Geobacter Aggregates

doi:10.1021/acs.analchem.0c00653

	In Vivo Molecular Insights into Syntrophic Geobacter Aggregates
	Wei, Wenchao1,2,3 ; Plymale, Andrew 2; Zhu, Zihua 4; Ma, Xiang 5; Liu, Fanghua1 ; Yu, Xiao-Ying 2
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
	2020-08-04
卷号	92 期号:15 页码:10402-10411
DOI	10.1021/acs.analchem.0c00653
通讯作者	Liu, Fanghua(fhliu@yic.ac.cn) ; Yu, Xiao-Ying(xiaoying.yu@pnnl.gov)
英文摘要	Direct interspecies electron transfer (DIET) has been considered as a novel and highly efficient strategy in both natural anaerobic environments and artificial microbial fuel cells. A syntrophic model consisting of Geobacter metallireducens and Geobacter sulfurreducens was studied in this work. We conducted in vivo molecular mapping of the outer surface of the syntrophic community as the interface of nutrients and energy exchange. System for Analysis at the Liquid Vacuum Interface combined with time-of-flight secondary ion mass spectrometry was employed to capture the molecular distribution of syntrophic Geobacter communities in the living and hydrated state. Principal component analysis with selected peaks revealed that syntrophic Geobacter aggregates were well differentiated from other control samples, including syntrophic planktonic cells, pure cultured planktonic cells, and single population biofilms. Our in vivo imaging indicated that a unique molecular surface was formed. Specifically, aromatic amino acids, phosphatidylethanolamine components, and large water clusters were identified as key components that favored the DIET of syntrophic Geobacter aggregates. Moreover, the molecular changes in depths of the Geobacter aggregates were captured using dynamic depth profiling. Our findings shed new light on the interface components supporting electron transfer in syntrophic communities based on in vivo molecular imaging.
资助机构	Pacific Northwest National Laboratory (PNNL) Earth and Biological Sciences Directorate (EBSD) mission seed Laboratory Directed Research and Development (LDRD) ; Yantai Institute of the Coastal Zone Research Chinese Academy of Sciences from the National Natural Science Foundation of China ; PNNL Alternate Sponsored Fellowship (ASF) ; Graduate Fellowship from University of Chinese Academy of Sciences ; DOE
收录类别	SCI
语种	英语
关键词[WOS]	ION MASS-SPECTROMETRY ; C-TYPE CYTOCHROME ; INTERSPECIES ELECTRON-TRANSFER ; TOF-SIMS ANALYSIS ; PROTEIN FILMS ; FATTY-ACIDS ; SURFACE ; BIOFILM ; PILI ; XPS
研究领域[WOS]	Chemistry
WOS记录号	WOS:000558761500031
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/28546
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Liu, Fanghua; Yu, Xiao-Ying
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China 2.Pacific Northwest Natl Lab, Energy & Environm Directorate, Richland, WA 99352 USA 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Pacific Northwest Natl Lab, Environm & Mol Sci Lab, Richland, WA 99352 USA 5.Grand View Univ, Dept Chem, Des Moines, IA 50316 USA
推荐引用方式 GB/T 7714	Wei, Wenchao,Plymale, Andrew,Zhu, Zihua,et al. In Vivo Molecular Insights into Syntrophic Geobacter Aggregates[J]. ANALYTICAL CHEMISTRY,2020,92(15):10402-10411.
APA	Wei, Wenchao,Plymale, Andrew,Zhu, Zihua,Ma, Xiang,Liu, Fanghua,&Yu, Xiao-Ying.(2020).In Vivo Molecular Insights into Syntrophic Geobacter Aggregates.ANALYTICAL CHEMISTRY,92(15),10402-10411.
MLA	Wei, Wenchao,et al."In Vivo Molecular Insights into Syntrophic Geobacter Aggregates".ANALYTICAL CHEMISTRY 92.15(2020):10402-10411.

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