| SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing | |
Bi, Liyan1; Wang, Xiao1; Cao, Xiaowei2; Liu, Luying1; Bai, Congcong1; Zheng, Qingyin1; Choo, Jaebum4; Chen, Lingxin1,3,5
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| 发表期刊 | TALANTA
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| ISSN | 0039-9140 |
| 2020-12-01 | |
| 卷号 | 220页码:8 |
| 关键词 | Au@AgNR SERS nanotag Antibiotic susceptibility testing Minimum inhibitory concentration Principal component analysis |
| DOI | 10.1016/j.talanta.2020.121397 |
| 通讯作者 | Bi, Liyan(liyan_bi@163.com) ; Choo, Jaebum(jbchoo@cau.ac.kr) ; Chen, Lingxin(lxchen@yic.ac.cn) |
| 英文摘要 | There is a challenge to obtain an ultrasensitive and rapid approach to detect bacteria and identify resistance. As a powerful bioanalytical tool, surface-enhanced Raman scattering (SERS) in bacterial detection have attracted increasing attentions. Herein, we developed a SERS-active Au@Ag core-shell nanorod (Au@AgNR) tag platform for ultrasensitive bacteria detection and antibiotic-susceptibility testing (AST). The platform established that surface enhanced Raman scattered Rhodamine 6G (R6G) absorption at 1517 cm(-1) had a good linearity (RI = 3865 + 193logC; R-2 = 0.97) with logarithm of E. coli concentration over a range of 10(7)-10(2) CFU (colony forming unit)/mL with limit of detection as low 10(2) CFU/mL. When E. coli was exposed to ampicillin at minimum inhibitory concentration (MIC, 4 mu g/mL), Raman spectroscopy showed the obvious variation between ampicillin-susceptible E. coli (Amp(-)-E. coli) and the ampicillin-resistant E. coli (Amp(+)-E. coli). Combined with principal component analysis (PCA) statistical analysis, the Raman intensity variation mentioned above allows to obtain rapid antibiotic resistance testing (<3.5 h). In addition, E.coli spiked into blood from C57BL/6 mice can be identified clearly, indicating the potential for point-of-care diagnostics. |
| 资助机构 | National Natural Science Foundation of China ; National Research Foundation of Korea ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ENHANCED RAMAN-SCATTERING ; SENSITIVE DETECTION ; GOLD ; IDENTIFICATION ; SPECTROSCOPY ; DESIGN ; PROBE ; ARRAY |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000571477400006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/28397 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Bi, Liyan; Choo, Jaebum; Chen, Lingxin |
| 作者单位 | 1.Binzhou Med Univ, Transformat Otol & Neurosci Ctr, Yantai 264003, Peoples R China 2.Yangzhou Univ, Med Coll, Inst Translat Med, Yangzhou 225001, Jiangsu, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 4.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bi, Liyan,Wang, Xiao,Cao, Xiaowei,et al. SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing[J]. TALANTA,2020,220:8. |
| APA | Bi, Liyan.,Wang, Xiao.,Cao, Xiaowei.,Liu, Luying.,Bai, Congcong.,...&Chen, Lingxin.(2020).SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing.TALANTA,220,8. |
| MLA | Bi, Liyan,et al."SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing".TALANTA 220(2020):8. |
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| SERS-active Au@Ag co（6976KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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