大叶藻(Zostera marina)海草床沉积物细菌和古菌丰度及组成的垂直剖面特征
Alternative TitleVertical patterns of bacterial and archaeal abundance and community structure in seagrass(Zostera marina) meadow sediments
郑鹏飞1; 张晓黎1; 龚骏1
Source Publication微生物学通报
ISSN0253-2654
2020
Volume47.0Issue:006Pages:1662-1674
Keyword海草床 垂直剖面 微生物群落结构 丰度 多样性
Abstract【背景】海草床是重要的"蓝碳"生态系统,对全球碳汇有重要贡献。海草床沉积物剖面的垂直梯度特征显著,表层呈现氧化态,富含活性有机质,而深层呈还原态,以惰性有机质为主。【目的】探究这种垂直特征如何影响微生物的丰度和群落分布。【方法】利用荧光定量PCR和16SrRNA基因高通量测序等手段,测定了山东荣成天鹅湖大叶藻海草床不同深度(5、10、15、20、25和30 cm)沉积物中细菌和古菌丰度、多样性和群落结构的变化。【结果】细菌和古菌16S rRNA基因拷贝数随深度的增加而降低,在沉积物5cm深处,细菌的16SrRNA基因拷贝数显著高于20cm和30cm层(ANOVA,P 0.05)。细菌中相对丰度最高的是变形菌门,其次是绿弯菌门,拟杆菌门,浮霉菌门等,其中δ-变形菌和浮霉菌的相对丰度随深度显著增加(P 0.05)。【结论】海草床沉积物细菌和古菌的丰度、多样性和群落分布具有明显的垂直特征,这种特征可能受沉积物有机质组成和氧化还原状况影响。
Other AbstractBackgroundThe seagrass meadow was a"Blue carbon"ecosystem contributing greatly to global carbon sequestration in coastal sediments.The vertical profile of seagrass sediments exhibits a strong redox gradient,where the surface layer was oxic and rich in labile organic matters and the deeper layer was reduced and dominated by recalcitrant organic matters.ObjectiveWe hypothesized that bacterial and archaeal communities varied greatly in abundance and community structure along the vertical gradient in the seagrass sediments.MethodsQuantitative real-time PCR and high-throughput sequencing were applied to characterize the prokaryotic communities at different sediment depths(5,10,15,20,25 and 30 cm)in the Zostera marina dominated meadow.ResultsThe 16 S rRNA gene copy numbers of bacteria and archaea decreased with the increasing sediment depth,and the bacterial copy numbers in the 5-cm layer was significantly higher than those in the 20-cm and 30-cm layers(ANOVA test,P 0.05).The most dominant bacterial phylum was Proteobacteria,followed by Chloroflexi,Bacteroidetes,and Planctomycetes.The relative abundances ofδ-Proteobacteria and Planctomycetes were significantly increased with increasing depth(P<0.05).Bathyarchaeota was the most dominant archaeal phylum,accounting for 70%in the 25-cm layer.Other abundant archaeal phyla were Woesearchaeota,Lokiarchaeota,Euryarchaeota and Thaumarchaeota.The relative abundance of Thaumarchaeota decreased significantly with increasing depth(P<0.05).ConclusionThe benthic archaeal and bacterial communities in the seagrass meadow sediments exhibited obvious vertical characteristic,which could be driven by organic matter composition and sediment redox status.
Indexed ByCSCD
Language中文
CSCD IDCSCD:6752564
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Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/28156
Collection中国科学院海岸带环境过程与生态修复重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Affiliation1.中国科学院海岸带环境过程与生态修复重点实验室
2.中国科学院大学
3.中山大学
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郑鹏飞,张晓黎,龚骏. 大叶藻(Zostera marina)海草床沉积物细菌和古菌丰度及组成的垂直剖面特征[J]. 微生物学通报,2020,47.0(006):1662-1674.
APA 郑鹏飞,张晓黎,&龚骏.(2020).大叶藻(Zostera marina)海草床沉积物细菌和古菌丰度及组成的垂直剖面特征.微生物学通报,47.0(006),1662-1674.
MLA 郑鹏飞,et al."大叶藻(Zostera marina)海草床沉积物细菌和古菌丰度及组成的垂直剖面特征".微生物学通报 47.0.006(2020):1662-1674.
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