Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis | |
Lu, Jiao1; Li, Zan1; Gao, Qing1; Tan, Jiangkun1; Sun, Zhiwei1; Chen, Lingxin1,2; You, Jinmao1,2 | |
发表期刊 | ANALYTICAL CHEMISTRY |
ISSN | 0003-2700 |
2021-02-23 | |
卷号 | 93期号:7页码:3426-3435 |
DOI | 10.1021/acs.analchem.0c04512 |
通讯作者 | Li, Zan(lilizanzan@163.com) ; Chen, Lingxin(lxchen@yic.ac.cn) ; You, Jinmao(jmyou6304@163.com) |
英文摘要 | Phagocyte respiratory burst in immune responses generates enormous amounts of reactive oxygen species (ROS) to fulfill primary defense against neoplasia. However, the beneficial functions associated with ROS, especially the potent oxidant/nucleophile peroxynitrite, in an immunological process are still ambiguous. Herein, we report the construction and biological assessment of cyanine-based fluorescent biosensors, which are based on a nonoxidative strategy for peroxynitrite detection. The established nonoxidative strategy is composed of nucleophilic substitution and nanoaggregate formation initiated by peroxynitrite. The proposed nonoxidative strategy in this study could maintain cellular oxidative stress in the critical process of detection and preserve homeostasis of cell metabolism. The remarkable detection sensitivity, reaction selectivity, and spectral photostability of our biosensors enabled us to visualize endogenous peroxynitrite levels in immune-stimulated phagocytes. With the aid of basal peroxynitrite imaging in an acute peritonitis model, the visualization of peroxynitrite level variations in immune responses of tumorigenesis was accomplished assisted by our biosensors. It is envisioned that our strategy provides a promising tool for early tumor diagnosis and evaluation of tumor suppression in the process of immune responses without disturbing the functions of ROS signaling transduction. |
资助机构 | National Natural Science Foundation of China ; Youth Program of Natural Science Foundation of Shandong Province |
收录类别 | SCI |
语种 | 英语 |
研究领域[WOS] | Chemistry |
WOS记录号 | WOS:000623041000014 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/27369 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
通讯作者 | Li, Zan; Chen, Lingxin; You, Jinmao |
作者单位 | 1.Qufu Normal Univ, Sch Chem & Chem Engn, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
推荐引用方式 GB/T 7714 | Lu, Jiao,Li, Zan,Gao, Qing,et al. Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis[J]. ANALYTICAL CHEMISTRY,2021,93(7):3426-3435. |
APA | Lu, Jiao.,Li, Zan.,Gao, Qing.,Tan, Jiangkun.,Sun, Zhiwei.,...&You, Jinmao.(2021).Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis.ANALYTICAL CHEMISTRY,93(7),3426-3435. |
MLA | Lu, Jiao,et al."Nonoxidative Strategy for Monitoring Peroxynitrite Fluctuations in Immune Responses of Tumorigenesis".ANALYTICAL CHEMISTRY 93.7(2021):3426-3435. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Nonoxidative Strateg(1894KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论