| FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant | |
Liu, Wei1,2,3 ; Bai, Xue1; Li, Yan1,2,3; Zhang, Haikun1,2,3; Hu, Xiaoke1,2,3
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| 发表期刊 | BMC MICROBIOLOGY
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| ISSN | 1471-2180 |
| 2021-03-09 | |
| 卷号 | 21期号:1页码:9 |
| 关键词 | Two-component response regulator REC domain HTH_LuxR domain Bacterial motility Exopolysaccharides Competitive nodulation |
| DOI | 10.1186/s12866-021-02138-w |
| 通讯作者 | Hu, Xiaoke(xkhu@yic.ac.cn) |
| 英文摘要 | BackgroundA wide variety of bacterial adaptative responses to environmental conditions are mediated by signal transduction pathways. Two-component signal transduction systems are one of the predominant means used by bacteria to sense the signals of the host plant and adjust their interaction behaviour. A total of seven open reading frames have been identified as putative two-component response regulators in the gram-negative nitrogen-fixing bacteria Azorhizobium caulinodans ORS571. However, the biological functions of these response regulators in the symbiotic interactions between A. caulinodans ORS571 and the host plant Sesbania rostrata have not been elucidated to date.ResultsIn this study, we identified and investigated a two-component response regulator, AcfR, with a phosphorylatable N-terminal REC (receiver) domain and a C-terminal HTH (helix-turn-helix) LuxR DNA-binding domain in A. caulinodans ORS571. Phylogenetic analysis showed that AcfR possessed close evolutionary relationships with NarL/FixJ family regulators. In addition, six histidine kinases containing HATPase_c and HisKA domains were predicted to interact with AcfR. Furthermore, the biological function of AcfR in free-living and symbiotic conditions was elucidated by comparing the wild-type strain and the Delta acfR mutant strain. In the free-living state, the cell motility behaviour and exopolysaccharide production of the Delta acfR mutant were significantly reduced compared to those of the wild-type strain. In the symbiotic state, the Delta acfR mutant showed a competitive nodule defect on the stems and roots of the host plant, suggesting that AcfR can provide A. caulinodans with an effective competitive ability for symbiotic nodulation.ConclusionsOur results showed that AcfR, as a response regulator, regulates numerous phenotypes of A. caulinodans under the free-living conditions and in symbiosis with the host plant. The results of this study help to elucidate the involvement of a REC+HTH_LuxR two-component response regulator in the Rhizobium-host plant interaction. |
| 资助机构 | National Natural Science Foundation of China |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PROTEIN ; SYSTEM |
| 研究领域[WOS] | Microbiology |
| WOS记录号 | WOS:000627445700002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/27366 |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Hu, Xiaoke |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Wei,Bai, Xue,Li, Yan,et al. FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant[J]. BMC MICROBIOLOGY,2021,21(1):9. |
| APA | Liu, Wei,Bai, Xue,Li, Yan,Zhang, Haikun,&Hu, Xiaoke.(2021).FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant.BMC MICROBIOLOGY,21(1),9. |
| MLA | Liu, Wei,et al."FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant".BMC MICROBIOLOGY 21.1(2021):9. |
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