Microbial degradation kinetics and molecular mechanism of 2,6-dichloro-4-nitrophenol by a Cupriavidus strain
Min, Jun; Xu, Lingxue; Fang, Suyun; Chen, Weiwei; Hu, Xiaoke
Source PublicationENVIRONMENTAL POLLUTION
2020-03
Volume258Pages:113703
KeywordBACILLUS-THERMOGLUCOSIDASIUS A7 AROMATIC HALOGENATED DBPS DISINFECTION BY-PRODUCTS P-NITROPHENOL PSEUDOMONAS-STUTZERI GENE-CLUSTER 2,4,6-TRICHLOROPHENOL DEGRADATION CATABOLISM PATHWAY CONTAMINATED SOIL BIODEGRADATION
MOST Discipline CatalogueEnvironmental Sciences
DOI10.1016/j.envpol.2019.113703
Contribution Rank[Min, Jun; Xu, Lingxue; Fang, Suyun; Chen, Weiwei; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; [Min, Jun; Fang, Suyun; Chen, Weiwei; Hu, Xiaoke] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China; [Min, Jun; Hu, Xiaoke] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China; [Xu, Lingxue] Yantai Univ, Coll Life Sci, Yantai, Peoples R China
Department海岸带生物学与生物资源保护实验室
Abstract2,6-Dichloro-4-nitrophenol (2,6-DCNP) is an emerging chlorinated nitroaromatic pollutant, and its fate in the environment is an important question. However, microorganisms with the ability to utilize 2,6-DCNP have not been reported. In this study, Cupriavidus sp. CNP-8 having been previously reported to degrade various halogenated nitrophenols, was verified to be also capable of degrading 2,6-DCNP. Biodegradation kinetics assay showed that it degraded 2,6-DCNP with the specific growth rate of 0.124 h(-1), half saturation constant of 0.038 mM and inhibition constant of 0.42 mM. Reai-time quantitative PCR analyses indicated that the hnp gene cluster was involved in the catabolism of 2,6-DCNP. The hnpA and hnpB gene products were purified to homogeneity by Ni-NTA chromatography. Enzymatic assays showed that HnpAB, a FAD-dependent two-component monooxygenase, converted 2,6-DCNP to 6-chlorohydroxyquinol with a K-m of 3.9 +/- 1.4 mu M and a k(cat)/K-m of 0.12 +/- 0.04 mu M-1 min(-1). As the oxygenase component encoding gene, hnpA is necessary for CNP-8 to grow on 2,6-DCNP by gene knockout and complementation. The phylogenetic analysis showed that the hnp cluster originated from the cluster involved in the catabolism of chlorophenols rather than nitrophenols. To our knowledge, CNP-8 is the first bacterium with the ability to utilize 2,6-DCNP, and this study fills a gap in the microbial degradation mechanism of this pollutant at the molecular, biochemical and genetic levels. Moreover, strain CNP-8 could degrade three chlorinated nitrophenols rapidly from the synthetic wastewater, indicating its potential in the bioremediation of chlorinated nitrophenols polluted environments. (C) 2019 Elsevier Ltd. All rights reserved.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordBACILLUS-THERMOGLUCOSIDASIUS A7 ; AROMATIC HALOGENATED DBPS ; DISINFECTION BY-PRODUCTS ; P-NITROPHENOL ; PSEUDOMONAS-STUTZERI ; GENE-CLUSTER ; 2,4,6-TRICHLOROPHENOL DEGRADATION ; CATABOLISM PATHWAY ; CONTAMINATED SOIL ; BIODEGRADATION
WOS IDWOS:000519655100130
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China [31600085] ; State's Key Project of Research and Development Plan [2016YFC1402300] ; Foreword Key Priority Research Program of Chinese Academy of Sciences [QYZDB-SSW-DQC013] ; Youth Innovation Promotion Association Program of the Chinese Academy of Sciences ; Yantai Science and Technology Project [2017ZH092]
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/25079
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China;
4.Yantai Univ, Coll Life Sci, Yantai, Peoples R China
Recommended Citation
GB/T 7714
Min, Jun,Xu, Lingxue,Fang, Suyun,et al. Microbial degradation kinetics and molecular mechanism of 2,6-dichloro-4-nitrophenol by a Cupriavidus strain[J]. ENVIRONMENTAL POLLUTION,2020,258:113703.
APA Min, Jun,Xu, Lingxue,Fang, Suyun,Chen, Weiwei,&Hu, Xiaoke.(2020).Microbial degradation kinetics and molecular mechanism of 2,6-dichloro-4-nitrophenol by a Cupriavidus strain.ENVIRONMENTAL POLLUTION,258,113703.
MLA Min, Jun,et al."Microbial degradation kinetics and molecular mechanism of 2,6-dichloro-4-nitrophenol by a Cupriavidus strain".ENVIRONMENTAL POLLUTION 258(2020):113703.
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