Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin
Wang, Xiaoyan; Yu, Shuangmei; Wang, Jiangru; Yu, Jialuo; Arabi, Maryam; Fu, Longwen; Li, Bowei; Li, Jinhua; Chen, Lingxin
Source PublicationTALANTA
2020-05-01
Volume211Pages:120727
KeywordRATIOMETRIC FLUORESCENCE OPTICAL-DETECTION GLYCOPROTEIN AFFINITY RECOGNITION SEPARATION STRATEGY SENSOR PH
MOST Discipline CatalogueChemistry, Analytical
DOI10.1016/j.talanta.2020.120727
Contribution Rank[Wang, Xiaoyan; Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Wang, Jiangru; Yu, Jialuo; Arabi, Maryam; Fu, Longwen; Li, Bowei; Li, Jinhua; Chen, Lingxin] Chinese Cademy Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Yu, Shuangmei] Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China
Department海岸带环境工程技术研究与发展中心
AbstractWe reported a facile strategy to assemble a ratiometric nanosensor for the ovalbumin (OVA) fluorescence determination and meanwhile it can be utilized for selective visual identification by naked eyes with fluorescent test papers under 365 nm UV lamp. The nanosensor was prepared through simply mixing blue color carbon dots (CDs) and green color core-shell imprinted polymers. Blue CDs were used directly as the internal reference without participating in the imprinting process and modified molecularly imprinted polymers (MIPs) were synthesized by post-imprinting, using fluorescein isothiocyanate (FITC) as fluorescence enhanced signal. Upon the addition of different concentrations of OVA, the fluorescence intensity of FITC was enhanced, while the fluorescence intensity of CDs was almost unchanged, leading to a detection limit as low as 15.4 nM. Accordingly, the fluorescence color was gradually changed from blue to dark olive green to green with naked eyes observation. Moreover, the ratiometric nanosensor was successfully applied to detect OVA in the human urine samples with satisfactory recoveries attaining of 92.0-104.0% with relative standard deviation (RSD) of 3.3-3.9% and 93.3-101.0% with RSDs of 2.7-3.8% for the spiked chicken egg white samples. This strategy reported here opens a novel pathway for biomacromolecule detection in real applications and can realize the visual observation on fluorescent test papers.
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China [21804010, 21575159, 41776110, 21876199, 21976209] ; Science and Technology Development Plan of Shandong Province of China [2014GGF01074] ; Chinese Academy of Sciences President's International Fellowship Initiative [2019PC0050] ; Research Initiation Fund of Binzhou Medical University [BY2019KYQD39] ; Taishan Scholar Project Special Funding [ts20190962]
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordRATIOMETRIC FLUORESCENCE ; OPTICAL-DETECTION ; GLYCOPROTEIN ; AFFINITY ; RECOGNITION ; SEPARATION ; STRATEGY ; SENSOR ; PH
WOS IDWOS:000518708300036
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/25039
Collection中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
Affiliation1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China;
2.Chinese Cademy Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
3.Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xiaoyan,Yu, Shuangmei,Wang, Jiangru,et al. Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin[J]. TALANTA,2020,211:120727.
APA Wang, Xiaoyan.,Yu, Shuangmei.,Wang, Jiangru.,Yu, Jialuo.,Arabi, Maryam.,...&Chen, Lingxin.(2020).Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin.TALANTA,211,120727.
MLA Wang, Xiaoyan,et al."Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin".TALANTA 211(2020):120727.
Files in This Item: Download All
File Name/Size DocType Version Access License
Fluorescent nanosens(1636KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Xiaoyan]'s Articles
[Yu, Shuangmei]'s Articles
[Wang, Jiangru]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Xiaoyan]'s Articles
[Yu, Shuangmei]'s Articles
[Wang, Jiangru]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Xiaoyan]'s Articles
[Yu, Shuangmei]'s Articles
[Wang, Jiangru]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.