| Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium | |
Min, J ; Xu, LX; Fang, SY; Chen, WW; Hu, XK
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| Source Publication | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| ISSN | 0175-7598 |
| 2019-09 | |
| Volume | 103Issue:18Pages:7741-7750 |
| Keyword | 1 2 4-Benzenetriol pathway 2-Chloro-4-nitrophenol Catabolic mechanism Gram-negative bacterium hnp cluster Two-component monooxygenase |
| MOST Discipline Catalogue | Biotechnology & Applied Microbiology |
| DOI | 10.1007/s00253-019-09994-7 |
| Contribution Rank | [Min, Jun; Xu, Lingxue; Fang, Suyun; Chen, Weiwei; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; [Min, Jun; Fang, Suyun; Chen, Weiwei; Hu, Xiaoke] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China; [Xu, Lingxue] Yantai Univ, Coll Life Sci, Yantai, Peoples R China |
| Department | 海岸带生物学与生物资源保护实验室 |
| Abstract | 2-Chloro-4-nitrophenol (2C4NP) is the most common chlorinated nitrophenol pollutant, and its environmental fate is of great concern. Cupriavidus sp. CNP-8, a Gram-negative bacterium, has been reported to degrade 2C4NP via the 1,2,4-benzenetriol (BT) pathway, significantly different from the (chloro)hydroquinone pathways reported in all other Gram-negative 2C4NP-utilizers. Herein, the BT pathway of the catabolism of 2C4NP in this strain was characterized at the molecular, biochemical, and genetic levels. The hnp gene cluster was suspected to be involved in the catabolism of 2C4NP because the hnp genes are significantly upregulated in the 2C4NP-induced strain CNP-8 compared to the uninduced strain. HnpAB, a two-component FAD-dependent monooxygenase, catalyzes the conversion of 2C4NP to BT via chloro-1,4-benzoquinone, with a K-m of 2.7 +/- 1.1 mu M and a k(cat)/K-m of 0.17 +/- 0.03 mu M-1 min(-1). hnpA is necessary for strain CNP-8 to utilize 2C4NP in vivo. HnpC, a BT 1,2-dioxygenase, was proved to catalyze BT ring-cleavage with formation of maleylacetate by HPLC-MS analysis. Phylogenetic analysis indicated that HnpA likely has different evolutionary origin compared to other functionally identified 2C4NP monooxygenases. To our knowledge, this is the first report revealing the catabolic mechanism of 2C4NP via the BT pathway in a Gram-negative bacterium, increasing our knowledge of the catabolic diversity for microbial 2C4NP degradation at the molecular and biochemical level. |
| Subtype | Article |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | GENE-CLUSTER ; PSEUDOMONAS-STUTZERI ; P-NITROPHENOL ; MONOOXYGENASE CATALYZES ; CATABOLISM PATHWAY ; STRAIN ; BIODEGRADATION ; 4-NITROPHENOL ; 2,4,6-TRICHLOROPHENOL ; IDENTIFICATION |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS ID | WOS:000483662400030 |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31600085] ; State's Key Project of Research and Development Plan [2016YFC1402300] ; Foreword Key Priority Research Program of Chinese Academy of Sciences [QYZDB-SSW-DQC013] ; Yantai Science and Technology Project [2017ZH092] ; State Key Laboratory of Microbial Metabolism, Shanghai JiaoTong University [MMLKF17-04] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/24871 |
| Collection | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| Affiliation | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China; 3.Yantai Univ, Coll Life Sci, Yantai, Peoples R China |
| Recommended Citation GB/T 7714 | Min, J,Xu, LX,Fang, SY,et al. Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2019,103(18):7741-7750. |
| APA | Min, J,Xu, LX,Fang, SY,Chen, WW,&Hu, XK.(2019).Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,103(18),7741-7750. |
| MLA | Min, J,et al."Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103.18(2019):7741-7750. |
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| Molecular and bioche（1032KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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