Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium
Min, J; Xu, LX; Fang, SY; Chen, WW; Hu, XK
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
2019-09
Volume103Issue:18Pages:7741-7750
Keyword1 2 4-Benzenetriol pathway 2-Chloro-4-nitrophenol Catabolic mechanism Gram-negative bacterium hnp cluster Two-component monooxygenase
MOST Discipline CatalogueBiotechnology & Applied Microbiology
DOI10.1007/s00253-019-09994-7
Contribution Rank[Min, Jun; Xu, Lingxue; Fang, Suyun; Chen, Weiwei; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; [Min, Jun; Fang, Suyun; Chen, Weiwei; Hu, Xiaoke] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China; [Xu, Lingxue] Yantai Univ, Coll Life Sci, Yantai, Peoples R China
Department海岸带生物学与生物资源保护实验室
Abstract2-Chloro-4-nitrophenol (2C4NP) is the most common chlorinated nitrophenol pollutant, and its environmental fate is of great concern. Cupriavidus sp. CNP-8, a Gram-negative bacterium, has been reported to degrade 2C4NP via the 1,2,4-benzenetriol (BT) pathway, significantly different from the (chloro)hydroquinone pathways reported in all other Gram-negative 2C4NP-utilizers. Herein, the BT pathway of the catabolism of 2C4NP in this strain was characterized at the molecular, biochemical, and genetic levels. The hnp gene cluster was suspected to be involved in the catabolism of 2C4NP because the hnp genes are significantly upregulated in the 2C4NP-induced strain CNP-8 compared to the uninduced strain. HnpAB, a two-component FAD-dependent monooxygenase, catalyzes the conversion of 2C4NP to BT via chloro-1,4-benzoquinone, with a K-m of 2.7 +/- 1.1 mu M and a k(cat)/K-m of 0.17 +/- 0.03 mu M-1 min(-1). hnpA is necessary for strain CNP-8 to utilize 2C4NP in vivo. HnpC, a BT 1,2-dioxygenase, was proved to catalyze BT ring-cleavage with formation of maleylacetate by HPLC-MS analysis. Phylogenetic analysis indicated that HnpA likely has different evolutionary origin compared to other functionally identified 2C4NP monooxygenases. To our knowledge, this is the first report revealing the catabolic mechanism of 2C4NP via the BT pathway in a Gram-negative bacterium, increasing our knowledge of the catabolic diversity for microbial 2C4NP degradation at the molecular and biochemical level.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordGENE-CLUSTER ; PSEUDOMONAS-STUTZERI ; P-NITROPHENOL ; MONOOXYGENASE CATALYZES ; CATABOLISM PATHWAY ; STRAIN ; BIODEGRADATION ; 4-NITROPHENOL ; 2,4,6-TRICHLOROPHENOL ; IDENTIFICATION
WOS Research AreaBiotechnology & Applied Microbiology
WOS IDWOS:000483662400030
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China [31600085] ; State's Key Project of Research and Development Plan [2016YFC1402300] ; Foreword Key Priority Research Program of Chinese Academy of Sciences [QYZDB-SSW-DQC013] ; Yantai Science and Technology Project [2017ZH092] ; State Key Laboratory of Microbial Metabolism, Shanghai JiaoTong University [MMLKF17-04]
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24871
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China;
3.Yantai Univ, Coll Life Sci, Yantai, Peoples R China
Recommended Citation
GB/T 7714
Min, J,Xu, LX,Fang, SY,et al. Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2019,103(18):7741-7750.
APA Min, J,Xu, LX,Fang, SY,Chen, WW,&Hu, XK.(2019).Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,103(18),7741-7750.
MLA Min, J,et al."Molecular and biochemical characterization of 2-chloro-4-nitrophenol degradation via the 1,2,4-benzenetriol pathway in a Gram-negative bacterium".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103.18(2019):7741-7750.
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