Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization | |
Zhang, XH; Lin, SJ; Liu, DY | |
发表期刊 | ENVIRONMENTAL MICROBIOLOGY |
ISSN | 1462-2912 |
2020-05 | |
卷号 | 22期号:5页码:9 |
关键词 | DISSOLVED ORGANIC PHOSPHORUS ALKALINE-PHOSPHATASE ACTIVITY RNA-SEQ DATA EXTRACELLULAR NUCLEOTIDES NITROGEN-FIXATION MARINE PHYTOPLANKTON LIMITATION OCEAN CYANOBACTERIUM |
研究领域 | Microbiology |
DOI | 10.1111/1462-2920.14944 |
产权排序 | [Zhang, Xiaohua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China; [Zhang, Xiaohua] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhang, Xiaohua] Binzhou Med Univ, Dept Gene Engn, Yantai 264003, Peoples R China; [Lin, Senjie] Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA; [Liu, Dongyan] East China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200241, Peoples R China |
通讯作者 | Liu, Dongyan(dyliu@sklec.ecnu.edu.cn) |
作者部门 | 海岸带环境过程实验室 |
英文摘要 | The capacity of phytoplankton to utilize dissolved organic phosphorus (DOP) plays an important role in their competition for resources when the availability of dissolved inorganic phosphorus (DIP) is low in the aquatic systems. Here, we explored the physiological and molecular responses of a globally distributed marine diatom, Skeletonema costatum, in utilizing adenosine-5 '-triphosphate (ATP) based on incubation experiments under ATP, DIP-replete, and DIP-depleted conditions. The results show that ATP supports the growth of S. costatum as efficiently as DIP. The pathway of S. costatum involved in utilizing ATP is not related to alkaline phosphatase (AP), an important DOP hydrolase, although extracellular hydrolysis is involved. The transcriptome analysis revealed several transcripts related to the hydrolase activity (e.g. NAD+ diphosphatase), which were significantly upregulated in the ATP culture group, indicating their possible involvement in ATP hydrolysis. Meanwhile, ATP-grown S. costatum exhibited downregulation of the genes related to a series of metabolic activities (e.g. purine metabolism), apparently to adapt to ATP condition. |
文章类型 | Article |
资助机构 | Natural Science Foundation of ChinaNational Natural Science Foundation of China [41876127, 41776116] ; Ministry of Science and Technology, ChinaMinistry of Science and Technology, China [2016YFE0133700, 2016YFA0600904] |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | DISSOLVED ORGANIC PHOSPHORUS ; ALKALINE-PHOSPHATASE ACTIVITY ; RNA-SEQ DATA ; EXTRACELLULAR NUCLEOTIDES ; NITROGEN-FIXATION ; MARINE ; PHYTOPLANKTON ; LIMITATION ; OCEAN ; CYANOBACTERIUM |
研究领域[WOS] | Microbiology |
WOS记录号 | WOS:000514890900001 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/24747 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Binzhou Med Univ, Dept Gene Engn, Yantai 264003, Peoples R China; 4.Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA; 5.East China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200241, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, XH,Lin, SJ,Liu, DY. Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization[J]. ENVIRONMENTAL MICROBIOLOGY,2020,22(5):9. |
APA | Zhang, XH,Lin, SJ,&Liu, DY.(2020).Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization.ENVIRONMENTAL MICROBIOLOGY,22(5),9. |
MLA | Zhang, XH,et al."Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization".ENVIRONMENTAL MICROBIOLOGY 22.5(2020):9. |
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