Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay | |
Lv, Enguang; Ding, Jiawang![]() ![]() | |
发表期刊 | ANALYTICAL CHEMISTRY
![]() |
ISSN | 0003-2700 |
2018-11-20 | |
卷号 | 90期号:22页码:13600-13606 |
关键词 | RAPID DETECTION MAGNETIC BEADS RECOGNITION MEMBRANE BACTERIOCINS BIOSENSOR SYSTEM TRIFLUOROETHANOL MECHANISMS ELECTRODES |
研究领域 | Chemistry |
DOI | 10.1021/acs.analchem.8b03809 |
产权排序 | [Lv, Enguang; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; [Lv, Enguang; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; [Ding, Jiawang; Qin, Wei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China; [Lv, Enguang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
作者部门 | 海岸带环境过程实验室 |
英文摘要 | Peptide-based sandwich assays are promising tools in molecular detection, but may be restricted by the availability of "pairs" of affinity peptides. Herein, a new potentiometric sandwich assay for bacteria based on peptide pairs derived from an antimicrobial peptide (AMP) ligand is demonstrated. As a model, the original AMP with a welldefined structure for Listeria monocytogenes (LM) can be split into two fragments to serve as the peptide pairs for the sandwich assay. The recognition and binding of the short peptide pairs to the target can be verified by circular dichroism, flow cytometry, fluorometry, and optical microscopy. The potentiometric magnetic bead-based sandwich assay is designed by using horseradish peroxidase as a label. The enzyme can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine with H2O2 to induce a potential change on a polymeric membrane ion selective electrode. Under optimal conditions, the concentration of LM can be determined potentiometrically in a linear range of 1.0 X 10(2) to 1.0 x 10(6) CFU mL(-1) with a detection limit of 10 CFU mL(-1) (3 sigma). The proposed sensing strategy expands the applications of peptides in the field of bioassays. |
文章类型 | Article |
资助机构 | National Natural Science Foundation of China [21575158, 21475148, 41876108] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020702] ; Taishan Scholar Program of Shandong Province [TS20081159] ; National Key Research and Development Program of China [2016YFC1400700] ; Key Research and Development Program of Yantai [2018ZHGY053] |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | RAPID DETECTION ; MAGNETIC BEADS ; RECOGNITION ; MEMBRANE ; BACTERIOCINS ; BIOSENSOR ; SYSTEM ; TRIFLUOROETHANOL ; MECHANISMS ; ELECTRODES |
研究领域[WOS] | Chemistry, Analytical |
WOS记录号 | WOS:000451246100062 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/24579 |
专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; 2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Lv, Enguang,Ding, Jiawang,Qin, Wei. Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay[J]. ANALYTICAL CHEMISTRY,2018,90(22):13600-13606. |
APA | Lv, Enguang,Ding, Jiawang,&Qin, Wei.(2018).Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay.ANALYTICAL CHEMISTRY,90(22),13600-13606. |
MLA | Lv, Enguang,et al."Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay".ANALYTICAL CHEMISTRY 90.22(2018):13600-13606. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Potentiometric Detec(2948KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论