Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay

doi:10.1021/acs.analchem.8b03809

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	Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay
	Lv, Enguang ; Ding, Jiawang; Qin, Wei
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
	2018-11-20
卷号	90 期号:22 页码:13600-13606
关键词	RAPID DETECTION MAGNETIC BEADS RECOGNITION MEMBRANE BACTERIOCINS BIOSENSOR SYSTEM TRIFLUOROETHANOL MECHANISMS ELECTRODES
研究领域	Chemistry
DOI	10.1021/acs.analchem.8b03809
产权排序	[Lv, Enguang; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; [Lv, Enguang; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; [Ding, Jiawang; Qin, Wei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China; [Lv, Enguang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	海岸带环境过程实验室
英文摘要	Peptide-based sandwich assays are promising tools in molecular detection, but may be restricted by the availability of "pairs" of affinity peptides. Herein, a new potentiometric sandwich assay for bacteria based on peptide pairs derived from an antimicrobial peptide (AMP) ligand is demonstrated. As a model, the original AMP with a welldefined structure for Listeria monocytogenes (LM) can be split into two fragments to serve as the peptide pairs for the sandwich assay. The recognition and binding of the short peptide pairs to the target can be verified by circular dichroism, flow cytometry, fluorometry, and optical microscopy. The potentiometric magnetic bead-based sandwich assay is designed by using horseradish peroxidase as a label. The enzyme can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine with H2O2 to induce a potential change on a polymeric membrane ion selective electrode. Under optimal conditions, the concentration of LM can be determined potentiometrically in a linear range of 1.0 X 10(2) to 1.0 x 10(6) CFU mL(-1) with a detection limit of 10 CFU mL(-1) (3 sigma). The proposed sensing strategy expands the applications of peptides in the field of bioassays.
文章类型	Article
资助机构	National Natural Science Foundation of China [21575158, 21475148, 41876108] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020702] ; Taishan Scholar Program of Shandong Province [TS20081159] ; National Key Research and Development Program of China [2016YFC1400700] ; Key Research and Development Program of Yantai [2018ZHGY053]
收录类别	SCI
语种	英语
关键词[WOS]	RAPID DETECTION ; MAGNETIC BEADS ; RECOGNITION ; MEMBRANE ; BACTERIOCINS ; BIOSENSOR ; SYSTEM ; TRIFLUOROETHANOL ; MECHANISMS ; ELECTRODES
研究领域[WOS]	Chemistry, Analytical
WOS记录号	WOS:000451246100062
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24579
专题	中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; 2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China; 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lv, Enguang,Ding, Jiawang,Qin, Wei. Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay[J]. ANALYTICAL CHEMISTRY,2018,90(22):13600-13606.
APA	Lv, Enguang,Ding, Jiawang,&Qin, Wei.(2018).Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay.ANALYTICAL CHEMISTRY,90(22),13600-13606.
MLA	Lv, Enguang,et al."Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay".ANALYTICAL CHEMISTRY 90.22(2018):13600-13606.

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