| PI3K signaling pathways modulated white spot syndrome virus (WSSV) replication in Procambarus clarkii | |
Zhang, Huijing; Yao, Xuemei; Ding, Yunfei; Xu, Zheng; Liang, Rongning ; Zhang, Ying; Wu, Yulong; Li, Boqing; Guan, Bo
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2018-05 | |
| 卷号 | 76页码:279-286 |
| 关键词 | PI3K WSSV replication Apoptosis Bax Bax inhibitor-1 Lectin Toll Procambarus clarkii |
| 研究领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2018.02.045 |
| 产权排序 | [Zhang, Huijing; Ding, Yunfei; Xu, Zheng; Zhang, Ying; Wu, Yulong; Li, Boqing] Binzhou Med Univ, Sch Basic Med Sci, Yantai 264003, Shandong, Peoples R China; [Zhang, Huijing; Yao, Xuemei] Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China; [Liang, Rongning; Guan, Bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China |
| 作者部门 | 海岸带环境过程实验室 |
| 英文摘要 | The PI3K/AKT signaling pathway is commonly exploited to regulate viral replication and affect the fate of infected cells. In the present study, a PI3K-specific inhibitor (LY294002) was employed to pretreat crayfish to evaluate the effects of PI3K/AKT signaling pathway in WSSV replication. The results showed that the WSSV copy numbers in crayfish pretreated with LY294002 were significantly lower than those in Tris-HCI pretreatment crayfish on the sixth and tenth day after WSSV infection. In semigranular cells, the apoptosis rates were up regulated on the third day post-WSSV infection, and a significantly lower proportion of apoptosis cells were observed in LY294002-pretreatment group. The expression level of Bax, Bax inhibitor-1 and lectin mRNA in haemocytes of crayfish were increased after WSSV infection. After the secondary stimulation with Tris-HCl, the Bax expression level in LY294002-pretreatment crayfish was significantly higher than that of crayfish pretreated with Tris-HCl on the third or sixth day, but the Toll and lectin mRNA expression decreased significantly on the third, sixth and tenth day. The Bax mRNA expression levels in LY294002-WSSV group were significantly higher than those in Tris-HCI-WSSV group on the third and tenth day. The Bax inhibitor-1 mRNA expression levels in LY294002-WSSV group were significantly lower than those in Tris-HCl-WSSV crayfish on the third day. These results together indicated that the hosts PI3K/AKT signaling pathway play positive roles in WSSV replication through the balance between host cell apoptois and innate immune responses. This information is helpful to further understand the role of PI3K/AKT signaling pathway on WSSV replication in Decapoda crustaceans. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [31402330, 41576106] ; Youth Prominent Teacher Training Plan of Binzhou Medical University ; Youth Innovation Promotion Association of CAS [2014190] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | RED SWAMP CRAYFISH ; L-TYPE LECTIN ; LITOPENAEUS-VANNAMEI ; VIRAL REPLICATION ; GENE-EXPRESSION ; CELL-DEATH ; APOPTOSIS ; INFECTION ; MACROPHAGES ; INVOLVEMENT |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000430888400034 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24471 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Binzhou Med Univ, Sch Basic Med Sci, Yantai 264003, Shandong, Peoples R China; 2.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China; 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Huijing,Yao, Xuemei,Ding, Yunfei,et al. PI3K signaling pathways modulated white spot syndrome virus (WSSV) replication in Procambarus clarkii[J]. FISH & SHELLFISH IMMUNOLOGY,2018,76:279-286. |
| APA | Zhang, Huijing.,Yao, Xuemei.,Ding, Yunfei.,Xu, Zheng.,Liang, Rongning.,...&Guan, Bo.(2018).PI3K signaling pathways modulated white spot syndrome virus (WSSV) replication in Procambarus clarkii.FISH & SHELLFISH IMMUNOLOGY,76,279-286. |
| MLA | Zhang, Huijing,et al."PI3K signaling pathways modulated white spot syndrome virus (WSSV) replication in Procambarus clarkii".FISH & SHELLFISH IMMUNOLOGY 76(2018):279-286. |
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