Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp strain CNP-8
Min, Jun; Wang, Jinpei; Chen, Weiwei; Hu, Xiaoke
Source PublicationAMB EXPRESS
ISSN2191-0855
2018-03-20
Volume8Pages:43
Keyword2-Chloro-4-nitrophenol Hydroxyquinol pathway Kinetics Chemotaxis Cupriavidus sp strain CNP-8
MOST Discipline CatalogueBiotechnology & Applied Microbiology
DOI10.1186/s13568-018-0574-7
Contribution Rank[Min, Jun; Chen, Weiwei; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China; [Wang, Jinpei] Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Hubei, Peoples R China
Department海岸带生物学与生物资源保护实验室
AbstractCupriavidus sp. strain CNP-8 isolated from a pesticide-contaminated soil was able to utilize 2-chloro-4-nitrophenol (2C4NP) as a sole source of carbon, nitrogen and energy, together with the release of nitrite and chloride ions. It could degrade 2C4NP at temperatures from 20 to 40 degrees C and at pH values from 5 to 10, and degrade 2C4NP as high as 1.6 mM. Kinetics assay showed that biodegradation of 2C4NP followed Haldane substrate inhibition model, with the maximum specific growth rate (mu(max)) of 0.148/h, half saturation constant (K-s) of 0.022 mM and substrate inhibition constant (K-i) of 0.72 mM. Strain CNP-8 was proposed to degrade 2C4NP with hydroxyquinol (1,2,4-benzenetriol, BT) as the ring-cleavage substrate. The 2C4NP catabolic pathway in strain CNP-8 is significant from those reported in other Gram-negative 2C4NP utilizers. Enzymatic assay indicated that the monooxygenase initiating 2C4NP catabolism had different substrates specificity compared with previously reported 2C4NP monooxygenations. Capillary assays showed that strain CNP-8 exhibited metabolism-dependent chemotactic response toward 2C4NP at the optimum concentration of 0.5 mM with a maximum chemotaxis index of 37.5. Furthermore, microcosm studies demonstrated that strain CNP-8, especially the pre-induced cells, could remove 2C4NP rapidly from the 2C4NP-contaminated soil. Considering its adaptability to pH and temperature fluctuations and great degradation efficiency against 2C4NP, strain CNP-8 could be a promising candidate for the bioremediation of 2C4NP-contaminated sites.
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordPSEUDOMONAS-MENDOCINA KR1 ; GENE-CLUSTER ; NITROPHENOL MONOOXYGENASE ; CONTAMINATED SOIL ; DEGRADATION ; CATABOLISM ; BIOAUGMENTATION ; KINETICS ; WBC-3 ; 4-MONOOXYGENASE
WOS Research AreaBiotechnology & Applied Microbiology
WOS IDWOS:000427902000002
Funding OrganizationNational Natural Science Foundation of China [31600085] ; Foreword Key Priority Research Program of Chinese Academy of Sciences [QYZDB-SSW-DQC013] ; National Key Research Program of China [2016YFC1402300] ; State Key Laboratory of Microbial Metabolism, Shanghai JiaoTong University [MMLKF15-04] ; Yantai Science and Technology Project [2017YT06000325]
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24384
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China;
2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Min, Jun,Wang, Jinpei,Chen, Weiwei,et al. Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp strain CNP-8[J]. AMB EXPRESS,2018,8:43.
APA Min, Jun,Wang, Jinpei,Chen, Weiwei,&Hu, Xiaoke.(2018).Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp strain CNP-8.AMB EXPRESS,8,43.
MLA Min, Jun,et al."Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp strain CNP-8".AMB EXPRESS 8(2018):43.
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