| Evaluation of sulfane sulfur bioeffects via a mitochondria-targeting selenium-containing near-infrared fluorescent probe | |
Gao, Min; Wang, Rui; Yu, Fabiao; Chen, Lingxin
| |
| 发表期刊 | BIOMATERIALS
 |
| ISSN | 0142-9612 |
| 2018-04 | |
| 卷号 | 160页码:1-14 |
| 关键词 | Fluorescent probes Sulfane sulfur Mitochondria-targeting In vivo bioimaging Hypoxia stress |
| 研究领域 | Engineering, Biomedical ; Materials Science, Biomaterials |
| DOI | 10.1016/j.biomaterials.2018.01.011 |
| 产权排序 | [Gao, Min; Wang, Rui; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Gao, Min] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 作者部门 | 海岸带环境工程技术研究与发展中心 |
| 英文摘要 | As a crucial member in antioxidant regulatory systems, sulfane sulfur plays essential roles in cytoprotective mechanisms by directly eliminating ROS and altering ROS-mediated redox signaling. Despite the rising interests in sulfane sulfur, there only a few bio-compatible methods are available for its direct detection. Moreover, most of the existing methods cannot meet the requirements of real-time detection due to the reactive and labile chemical properties of sulfane sulfur. Therefore, we strive to clarify the mutual relationship between mitochondria sulfane sulfur and ROS under hypoxia stress. Herein, we report a near-infrared fluorescent probe Mito-SeH for the selective imaging of mitochondrial sulfane sulfur in cells and in vivo under hypoxia stress. Mito-SeH includes three moieties: a selenol group (-SeH) as the stronger sulfur-acceptor; a near-infrared azo-BODIPY fluorophore as the fluorescent modulator; a lipophilic alkyltriphenylphosphonium cation as the mitochondrial delivery. Mito-SeH exhibits excellent selectivity and sensitivity towards the detection of mitochondria sulfane sulfur. The hypoxia response behavior of Mito-SeH is evaluated in monolayer cell and three-dimensional multicellular spheroid to clarify the relationship between sulfane sulfur and hypoxia. We confirm that sulfane sulfur protection mechanism against hypoxia is to inhibition of caspase-dependent apoptosis through directly scavenging ROS pathway. The probe is also applied to measurement of sulfane sulfur in ex vivo-dissected organs of hypoxic mouse model, as well as the probe is successfully used for real-time monitoring the changes of sulfane sulfur and ROS in acute ischemia mice model. We suggest that sulfane sulfur may be a novel therapeutic agent for hypoxia-induced injury. (C) 2018 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | National Nature Science Foundation of China [21405172, 21775162, 21575159, 21522706] ; program of Youth Innovation Promotion Association, CAS [2015170] ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | HYDROGEN-SULFIDE ; IN-VIVO ; 3-MERCAPTOPYRUVATE SULFURTRANSFERASE ; BIOLOGICAL-SYSTEMS ; OXIDATIVE STRESS ; CHEMICAL BIOLOGY ; REGULATORY ROLE ; LIVING CELLS ; HYPOXIA ; POLYSULFIDES |
| 研究领域[WOS] | Engineering ; Materials Science |
| WOS记录号 | WOS:000425573400001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24381 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Min,Wang, Rui,Yu, Fabiao,et al. Evaluation of sulfane sulfur bioeffects via a mitochondria-targeting selenium-containing near-infrared fluorescent probe[J]. BIOMATERIALS,2018,160:1-14. |
| APA | Gao, Min,Wang, Rui,Yu, Fabiao,&Chen, Lingxin.(2018).Evaluation of sulfane sulfur bioeffects via a mitochondria-targeting selenium-containing near-infrared fluorescent probe.BIOMATERIALS,160,1-14. |
| MLA | Gao, Min,et al."Evaluation of sulfane sulfur bioeffects via a mitochondria-targeting selenium-containing near-infrared fluorescent probe".BIOMATERIALS 160(2018):1-14. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Evaluation of sulfan（4841KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论