Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803
Chen, Gao1,2; Chen, Jun3,4; He, Qingfang5; Zhang, Yan1,2; Peng, Zhenying1,2; Fan, Zhongxue1,2; Bian, Fei1,2; Yu, Jinhui1,2; Qin, Song3; Chen, Gao(Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China); Qin, Song(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China)
2017-12-01
发表期刊ENERGIES
ISSN1996-1073
卷号10期号:12
产权排序第3完成单位
摘要Palmitoleic acid (C16:1) and stearic acid (C18:0) are precursors of polyunsaturated fatty acids, which are the focus of intensive global research due to their nutritional value, medicinal applications, and potential use as biofuel. Acyl-acyl carrier protein (ACP) thioesterases are intraplastidial enzymes that determine the types and amounts of fatty acids produced in plants and release fatty acids into the cytosol to be incorporated into glycerolipids. Based on amino acid sequence identity and substrate specificity, these enzymes are classified into two families, FatA and FatB. In this study, we cloned FatA and FatB thioesterases from Arachis hypogaea L. seeds and functionally expressed these genes, both individually and in tandem, in a blue-green alga Synechocystis sp. PCC6803. The heterologous expression of these genes in Synechocystis altered the fatty acid composition of lipids, resulting in a 29.5-31.6% increase in palmitoleic acid production and a 22.5-35.5% increase in stearic acid production. Moreover, the transgenic Synechocystis cells also showed significant increases in levels of oleic acid (C18: 1, OA), linoleic acid (C18: 2, LA), and alpha-linolenic acid (C18: 3n3, ALA). These results suggest that the fatty acid profile of algae can be significantly improved by the heterologous expression of exogenous genes. This study not only provides insight into fatty acid biosynthesis, but also lays the foundation for manipulating the fatty acid content of cyanobacteria.
关键词Fatty Acids Gene Expression Acyl-acyl Carrier Protein Thioesterases Synechocystis Sp Pcc6803
DOI10.3390/en10122093
项目资助者Natural Science Foundation of Shandong Province, China(ZR2016CM48) ; Young Talents Training Program of Shandong Academy of Agricultural Sciences ; China Postdoctoral Science Foundation(2014M551942) ; National Key Research and Development Program-China(2016YFF0202304)
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收录类别SCI
关键词[WOS]CARRIER PROTEIN THIOESTERASE ; BIODIESEL PRODUCTION ; TRANSGENIC PLANTS ; CYANOBACTERIA ; PURIFICATION ; MICROALGAE ; CLONING ; ALGAE ; SEEDS ; GENE
文章类型Article
语种英语
WOS研究方向Energy & Fuels
WOS记录号WOS:000423156900164
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/23566
专题海岸带生物学与生物资源利用所重点实验室
通讯作者Chen, Gao; Qin, Song; Chen, Gao(Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China); Qin, Song(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China)
作者单位1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
2.Shandong Prov Key Lab Genet Improvement Ecol & Ph, Jinan 250100, Shandong, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA
通讯作者单位中国科学院烟台海岸带研究所
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Chen, Gao,Chen, Jun,He, Qingfang,et al. Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803[J]. ENERGIES,2017,10(12).
APA Chen, Gao.,Chen, Jun.,He, Qingfang.,Zhang, Yan.,Peng, Zhenying.,...&Qin, Song.(2017).Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803.ENERGIES,10(12).
MLA Chen, Gao,et al."Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803".ENERGIES 10.12(2017).
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