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题名:
Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins
作者: Wang, Xiaoyan1,2,3; Yu, Jialuo2,3; Li, Jinhua2; Kang, Qi3; Shen, Dazhong3; Chen, Lingxin2
刊名: SENSORS AND ACTUATORS B-CHEMICAL
ISSN号: 0925-4005
出版日期: 2018-02-01
卷号: 255, 页码:268-274
关键词: Molecular imprinting ; Protein imprinting ; Fluorescent detection ; Phycocyanin ; Quantum dots
DOI: 10.1016/j.snb.2017.08.068
产权排序: 第2完成单位
通讯作者: Shen, Dazhong(Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China) ; Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
英文摘要: Molecular imprinting as a versatile technology has gained popularity in various fields for diverse species; however, protein imprinting still remains challenging due to some inherent problems but is potentially rewarding work. Herein, we reported a facile strategy to imprint phycocyanin (PC), as a template, using quantum dots (QDs) co-capped by thioglycollic acid and glutathione directly as functional monomer via dopamine self-polymerization, which effectively simplified the imprinting process and afforded easy accessibility to recognition sites. The resulting QDs based imprinting nanosensor showed significant fluorescence decrease of QDs within less than 16 s upon binding PC owing to the ultrathin imprinting layer (ca. 3 nm), leading to a high detectability down to 0.075 mu M. An excellent linearity was found within the wide range of 0.8-8.0 mu M (R-2 = 0.9935). Good recognition selectivity toward PC was displayed over other possible competing molecules, with a high imprinting factor of 7.3. Seawater and lake water samples spiked with PC were also analyzed, presenting satisfactory recoveries ranging from 90.8-110.1% with precisions below 4.6%, indicating the practicality of the imprinting nanosensor for accurately rapid nanoscale monitoring of algae blooms. By using another protein, bovine haemoglobin (BHb), as a convincing support model, the present study can provide a simple and general imprinting strategy toward various concerned proteins through rationally utilizing diverse functionalization and interactions. (C) 2017 Elsevier B.V. All rights reserved.
研究领域[WOS]: Chemistry ; Electrochemistry ; Instruments & Instrumentation
项目资助者: National Natural Science Foundation of China(21477160, 21575159, 21575080) ; Research Fund for the Doctoral Program of Higher Education of China(20113704110003) ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
关键词[WOS]: DOPED ZNS QDS ; RECOGNITION ; POLYMER ; TARGET ; SURFACE ; NANOPARTICLES ; MICROSPHERES ; SEPARATION ; SILICA ; CELLS
文章类型: Article
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000414151800032
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内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/23466
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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作者单位: 1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China

Recommended Citation:
Wang, Xiaoyan,Yu, Jialuo,Li, Jinhua,et al. Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,255:268-274.
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