Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins

doi:10.1016/j.snb.2017.08.068

	Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins
	Wang, Xiaoyan 1,2,3; Yu, Jialuo 2,3; Li, Jinhua2 ; Kang, Qi 3; Shen, Dazhong 3; Chen, Lingxin 2; Shen, Dazhong(Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
	2018-02-01
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
ISSN	0925-4005
卷号	255 页码:268-274
产权排序	第2完成单位
摘要	Molecular imprinting as a versatile technology has gained popularity in various fields for diverse species; however, protein imprinting still remains challenging due to some inherent problems but is potentially rewarding work. Herein, we reported a facile strategy to imprint phycocyanin (PC), as a template, using quantum dots (QDs) co-capped by thioglycollic acid and glutathione directly as functional monomer via dopamine self-polymerization, which effectively simplified the imprinting process and afforded easy accessibility to recognition sites. The resulting QDs based imprinting nanosensor showed significant fluorescence decrease of QDs within less than 16 s upon binding PC owing to the ultrathin imprinting layer (ca. 3 nm), leading to a high detectability down to 0.075 mu M. An excellent linearity was found within the wide range of 0.8-8.0 mu M (R-2 = 0.9935). Good recognition selectivity toward PC was displayed over other possible competing molecules, with a high imprinting factor of 7.3. Seawater and lake water samples spiked with PC were also analyzed, presenting satisfactory recoveries ranging from 90.8-110.1% with precisions below 4.6%, indicating the practicality of the imprinting nanosensor for accurately rapid nanoscale monitoring of algae blooms. By using another protein, bovine haemoglobin (BHb), as a convincing support model, the present study can provide a simple and general imprinting strategy toward various concerned proteins through rationally utilizing diverse functionalization and interactions. (C) 2017 Elsevier B.V. All rights reserved.
关键词	Molecular Imprinting Protein Imprinting Fluorescent Detection Phycocyanin Quantum Dots
DOI	10.1016/j.snb.2017.08.068
项目资助者	National Natural Science Foundation of China(21477160, 21575159, 21575080) ; Research Fund for the Doctoral Program of Higher Education of China(20113704110003) ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
URL	查看原文
收录类别	SCI
关键词[WOS]	DOPED ZNS QDS ; RECOGNITION ; POLYMER ; TARGET ; SURFACE ; NANOPARTICLES ; MICROSPHERES ; SEPARATION ; SILICA ; CELLS
文章类型	Article
语种	英语
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000414151800032
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/23466
专题	中科院海岸带环境过程与生态修复重点实验室
通讯作者	Shen, Dazhong; Chen, Lingxin; Shen, Dazhong(Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
作者单位	1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaoyan,Yu, Jialuo,Li, Jinhua,et al. Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,255:268-274.
APA	Wang, Xiaoyan.,Yu, Jialuo.,Li, Jinhua.,Kang, Qi.,Shen, Dazhong.,...&Chen, Lingxin.(2018).Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins.SENSORS AND ACTUATORS B-CHEMICAL,255,268-274.
MLA	Wang, Xiaoyan,et al."Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins".SENSORS AND ACTUATORS B-CHEMICAL 255(2018):268-274.