Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins
Shen, Dazhong(Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China)
; Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
英文摘要:
Molecular imprinting as a versatile technology has gained popularity in various fields for diverse species; however, protein imprinting still remains challenging due to some inherent problems but is potentially rewarding work. Herein, we reported a facile strategy to imprint phycocyanin (PC), as a template, using quantum dots (QDs) co-capped by thioglycollic acid and glutathione directly as functional monomer via dopamine self-polymerization, which effectively simplified the imprinting process and afforded easy accessibility to recognition sites. The resulting QDs based imprinting nanosensor showed significant fluorescence decrease of QDs within less than 16 s upon binding PC owing to the ultrathin imprinting layer (ca. 3 nm), leading to a high detectability down to 0.075 mu M. An excellent linearity was found within the wide range of 0.8-8.0 mu M (R-2 = 0.9935). Good recognition selectivity toward PC was displayed over other possible competing molecules, with a high imprinting factor of 7.3. Seawater and lake water samples spiked with PC were also analyzed, presenting satisfactory recoveries ranging from 90.8-110.1% with precisions below 4.6%, indicating the practicality of the imprinting nanosensor for accurately rapid nanoscale monitoring of algae blooms. By using another protein, bovine haemoglobin (BHb), as a convincing support model, the present study can provide a simple and general imprinting strategy toward various concerned proteins through rationally utilizing diverse functionalization and interactions. (C) 2017 Elsevier B.V. All rights reserved.
National Natural Science Foundation of China(21477160, 21575159, 21575080)
; Research Fund for the Doctoral Program of Higher Education of China(20113704110003)
; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China
Recommended Citation:
Wang, Xiaoyan,Yu, Jialuo,Li, Jinhua,et al. Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,255:268-274.
