Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins
Wang, Xiaoyan1,2,3; Yu, Jialuo2,3; Li, Jinhua2; Kang, Qi3; Shen, Dazhong3; Chen, Lingxin2; Shen, Dazhong(Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
2018-02-01
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
卷号255页码:268-274
产权排序第2完成单位
摘要Molecular imprinting as a versatile technology has gained popularity in various fields for diverse species; however, protein imprinting still remains challenging due to some inherent problems but is potentially rewarding work. Herein, we reported a facile strategy to imprint phycocyanin (PC), as a template, using quantum dots (QDs) co-capped by thioglycollic acid and glutathione directly as functional monomer via dopamine self-polymerization, which effectively simplified the imprinting process and afforded easy accessibility to recognition sites. The resulting QDs based imprinting nanosensor showed significant fluorescence decrease of QDs within less than 16 s upon binding PC owing to the ultrathin imprinting layer (ca. 3 nm), leading to a high detectability down to 0.075 mu M. An excellent linearity was found within the wide range of 0.8-8.0 mu M (R-2 = 0.9935). Good recognition selectivity toward PC was displayed over other possible competing molecules, with a high imprinting factor of 7.3. Seawater and lake water samples spiked with PC were also analyzed, presenting satisfactory recoveries ranging from 90.8-110.1% with precisions below 4.6%, indicating the practicality of the imprinting nanosensor for accurately rapid nanoscale monitoring of algae blooms. By using another protein, bovine haemoglobin (BHb), as a convincing support model, the present study can provide a simple and general imprinting strategy toward various concerned proteins through rationally utilizing diverse functionalization and interactions. (C) 2017 Elsevier B.V. All rights reserved.
关键词Molecular Imprinting Protein Imprinting Fluorescent Detection Phycocyanin Quantum Dots
DOI10.1016/j.snb.2017.08.068
项目资助者National Natural Science Foundation of China(21477160, 21575159, 21575080) ; Research Fund for the Doctoral Program of Higher Education of China(20113704110003) ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
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收录类别SCI
关键词[WOS]DOPED ZNS QDS ; RECOGNITION ; POLYMER ; TARGET ; SURFACE ; NANOPARTICLES ; MICROSPHERES ; SEPARATION ; SILICA ; CELLS
文章类型Article
语种英语
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000414151800032
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/23466
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Shen, Dazhong; Chen, Lingxin; Shen, Dazhong(Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China); Chen, Lingxin(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China)
作者单位1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China
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Wang, Xiaoyan,Yu, Jialuo,Li, Jinhua,et al. Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,255:268-274.
APA Wang, Xiaoyan.,Yu, Jialuo.,Li, Jinhua.,Kang, Qi.,Shen, Dazhong.,...&Chen, Lingxin.(2018).Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins.SENSORS AND ACTUATORS B-CHEMICAL,255,268-274.
MLA Wang, Xiaoyan,et al."Quantum dots based imprinting fluorescent nanosensor for the selective and sensitive detection of phycocyanin: A general imprinting strategy toward proteins".SENSORS AND ACTUATORS B-CHEMICAL 255(2018):268-274.
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