| Effect of inoculation of Burkholderia sp strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil | |
Min, Jun1 ; Wang, Bin1,2; Hu, Xiaoke1; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Shandong, Peoples R China)
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| Source Publication | SCIENTIFIC REPORTS
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| ISSN | 2045-2322 |
| 2017-07-20 | |
| Volume | 7Pages:5983 |
| Keyword | RALSTONIA SP SJ98 CONTAMINATED SOIL P-NITROPHENOL BIOAUGMENTATION DIVERSITY BIODEGRADATION BIOREMEDIATION 4-NITROPHENOL BIODIVERSITY CHEMOTAXIS |
| MOST Discipline Catalogue | Multidisciplinary Sciences |
| DOI | 10.1038/s41598-017-06436-0 |
| Contribution Rank | 第1完成单位 |
| Department | 海岸带生物学与生物资源保护实验室 |
| Abstract | para-Nitrophenol (PNP), 3-methyl-4-nitrophenol (3M4NP), and 2-chloro-4-nitrophenol (2C4NP) are highly toxic compounds that have caused serious environmental issues. We inoculated an artificially contaminated soil with Burkholderia sp. strain SJ98, which has the ability to degrade PNP, 3M4NP, and 2C4NP, and quantified bioremediation. There was accelerated degradation of all nitrophenols in inoculated treatments compared to the un-inoculated treatments. The indigenous bacteria were able to degrade PNP, but not 3M4NP or 2C4NP. Real-time PCR targeting the catabolic gene pnpA showed that levels of strain SJ98 remained stable over the incubation period. High-throughput sequencing revealed that both contamination and bioaugmentation influenced the bacterial community structure. Bioaugmentation seemed to protect Kineosporia, Nitrososphaera, and Schlesneria from nitrophenol inhibition, as well as led to a sharp increase in the abundance of Nonomuraea, Kribbella, and Saccharopolyspora. There was a significant increase in the relative abundances of Thermasporomyces, Actinomadura, and Streptomyces in both contaminated and bioaugmented treatments; this indicated that these bacteria are likely directly related to nitrophenol degradation. To our knowledge, this is the first report of the simultaneous removal of PNP, 3M4NP, and 2C4NP using bioaugmentation. This study provides valuable insights into the bioremediation of soils contaminated with nitrophenols. |
| Subtype | Article |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | RALSTONIA SP SJ98 ; CONTAMINATED SOIL ; P-NITROPHENOL ; BIOAUGMENTATION ; DIVERSITY ; BIODEGRADATION ; BIOREMEDIATION ; 4-NITROPHENOL ; BIODIVERSITY ; CHEMOTAXIS |
| WOS Research Area | Science & Technology - Other Topics |
| WOS ID | WOS:000405907800037 |
| Funding Organization | National Natural Science Foundation of China(31600085) ; National Key Research Program of China(2016YFC1402300) ; Chinese Academy of Sciences(QYZDB-SSW-DQC013) ; Yantai Science and Technology Project(2014ZH088) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/23413 |
| Collection | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| Corresponding Author | Hu, Xiaoke; Hu, Xiaoke(Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Shandong, Peoples R China) |
| Affiliation | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Min, Jun,Wang, Bin,Hu, Xiaoke,et al. Effect of inoculation of Burkholderia sp strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil[J]. SCIENTIFIC REPORTS,2017,7:5983. |
| APA | Min, Jun,Wang, Bin,Hu, Xiaoke,&Hu, Xiaoke.(2017).Effect of inoculation of Burkholderia sp strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil.SCIENTIFIC REPORTS,7,5983. |
| MLA | Min, Jun,et al."Effect of inoculation of Burkholderia sp strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil".SCIENTIFIC REPORTS 7(2017):5983. |
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