Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer

doi:10.1021/acsnano.7b01536

	Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer
	Cheng, Ziyi 1; Choi, Namhyun 1; Wang, Rui 1; Lee, Sangyeop 1; Moon, Kyung Chul 2; Yoon, Soo-Young 2; Chen, Lingxin3 ; Choo, Jaebum 1
发表期刊	ACS NANO
ISSN	1936-0851
	2017-05-01
卷号	11 期号:5 页码:4926-4933
关键词	Sers-based Immunoassay Multiplex Detection Prostate Cancer Free To Total Psa Ratio Simultaneous Detection
研究领域	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
DOI	10.1021/acsnano.7b01536
产权排序	[Cheng, Ziyi; Choi, Namhyun; Wang, Rui; Lee, Sangyeop; Choo, Jaebum] Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea; [Moon, Kyung Chul; Yoon, Soo-Young] Korea Univ, Dept Lab Med, Coll Med, Seoul 152854, South Korea; [Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
作者部门	海岸带环境工程技术研究与发展中心
英文摘要	Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.
文章类型	Article
资助机构	National Research Foundation of Korea(R11-2008-0061852 ; Nano Material Technology Development Program through the National Research Foundation of Korea ; Ministry of Education, Science, and Technology(2012035286) ; Korea Health Industry Development Institute (KHIDI) - Ministry of Health and Welfare, Korea(HI16C2129) ; National Natural Science Foundation of China(21575159) ; K20904000004-12A0500-00410)
收录类别	SCI
语种	英语
关键词[WOS]	TOTAL PSA ; BIOMARKER DETECTION ; SERS ; NANOPARTICLES ; MARKERS ; PROTEIN ; ASSAY ; DIFFERENTIATION ; NANOPROBES ; PERCENTAGE
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS记录号	WOS:000402498400061
引用统计	被引频次：291[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/22522
专题	中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea 2.Korea Univ, Dept Lab Med, Coll Med, Seoul 152854, South Korea 3.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Ziyi,Choi, Namhyun,Wang, Rui,et al. Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer[J]. ACS NANO,2017,11(5):4926-4933.
APA	Cheng, Ziyi.,Choi, Namhyun.,Wang, Rui.,Lee, Sangyeop.,Moon, Kyung Chul.,...&Choo, Jaebum.(2017).Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer.ACS NANO,11(5),4926-4933.
MLA	Cheng, Ziyi,et al."Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer".ACS NANO 11.5(2017):4926-4933.