Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer
Cheng, Ziyi1; Choi, Namhyun1; Wang, Rui1; Lee, Sangyeop1; Moon, Kyung Chul2; Yoon, Soo-Young2; Chen, Lingxin3; Choo, Jaebum1
2017-05-01
发表期刊ACS NANO
ISSN1936-0851
卷号11期号:5页码:4926-4933
摘要Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.
关键词Sers-based Immunoassay Multiplex Detection Prostate Cancer Free To Total Psa Ratio Simultaneous Detection
DOI10.1021/acsnano.7b01536
项目资助者National Research Foundation of Korea(R11-2008-0061852 ; Nano Material Technology Development Program through the National Research Foundation of Korea ; Ministry of Education, Science, and Technology(2012035286) ; Korea Health Industry Development Institute (KHIDI) - Ministry of Health and Welfare, Korea(HI16C2129) ; National Natural Science Foundation of China(21575159) ; K20904000004-12A0500-00410)
收录类别SCI
关键词[WOS]TOTAL PSA ; BIOMARKER DETECTION ; SERS ; NANOPARTICLES ; MARKERS ; PROTEIN ; ASSAY ; DIFFERENTIATION ; NANOPROBES ; PERCENTAGE
文章类型Article
语种英语
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS记录号WOS:000402498400061
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22522
专题中科院海岸带环境过程与生态修复重点实验室
作者单位1.Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea
2.Korea Univ, Dept Lab Med, Coll Med, Seoul 152854, South Korea
3.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Cheng, Ziyi,Choi, Namhyun,Wang, Rui,et al. Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer[J]. ACS NANO,2017,11(5):4926-4933.
APA Cheng, Ziyi.,Choi, Namhyun.,Wang, Rui.,Lee, Sangyeop.,Moon, Kyung Chul.,...&Choo, Jaebum.(2017).Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer.ACS NANO,11(5),4926-4933.
MLA Cheng, Ziyi,et al."Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer".ACS NANO 11.5(2017):4926-4933.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cheng, Ziyi]的文章
[Choi, Namhyun]的文章
[Wang, Rui]的文章
百度学术
百度学术中相似的文章
[Cheng, Ziyi]的文章
[Choi, Namhyun]的文章
[Wang, Rui]的文章
必应学术
必应学术中相似的文章
[Cheng, Ziyi]的文章
[Choi, Namhyun]的文章
[Wang, Rui]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。