A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation

doi:10.1002/adfm.201700769

	A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation
	Han, Xiaoyue 1,2; Song, Xinyu 1; Yu, Fabiao 1; Chen, Lingxin1
发表期刊	ADVANCED FUNCTIONAL MATERIALS
ISSN	1616-301X
	2017-07-26
卷号	27 期号:28 页码:1700769
关键词	Inflammation Near-infrared Fluorescent Probes Protective Effects Selenocysteine
研究领域	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
DOI	10.1002/adfm.201700769
产权排序	[Han, Xiaoyue; Song, Xinyu; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	海岸带环境工程技术研究与发展中心
英文摘要	Selenocysteine (Sec) is a primary kind of reactive selenium species in cells whose antioxidant roles in a series of liver diseases have been featured. However, it is difficult to determine Sec in living cells and in vivo due to its high reactivity and instability. This work reports a ratiometric near-infrared fluorescent probe (Cy-SS) for qualitative and quantitative determination of Sec in living cells and in vivo. The probe is composed of heptamethine cyanine fluorophore, the response unit bis(2-hydroxyethyl)disulfide, and the liver-targeting moiety D-galactose. Based on a detection mechanism of selenium-sulfur exchange reaction, the concentrations of Sec in HepG2, HL-7702 cells, and primary mouse hepatocytes is determined as 3.08 +/- 0.11 x 10(-6) M, 4.03 +/- 0.16 x 10(-6) M and 4.34 +/- 0.30 x 10(-6) M, respectively. The probe can selectively accumulate in liver. The ratio fluorescence signal of the probe can be employed to quantitatively analyze the fluctuation of Sec concentrations in cells and mice models of acute hepatitis. The experimental results demonstrate that Sec plays important antioxidant and anti-inflammatory roles during inflammatory process. And the levels of intracellular Sec have a close relationship with the degree of liver inflammation. The above imaging detections make this new probe a potential candidate for the accurate diagnosis of inflammation.
文章类型	Article
资助机构	National Nature Science Foundation of China(21405172 ; program of Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS(KF2016-22) ; 21575159 ; 21275158)
收录类别	SCI
语种	英语
关键词[WOS]	LIVING CELLS ; THIOREDOXIN REDUCTASE ; CARBON-TETRACHLORIDE ; SELENIUM COMPOUNDS ; LIVER-DAMAGE ; THIOLS ; SULFUR ; MECHANISMS ; TISSUES ; RATS
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS记录号	WOS:000406183100007
引用统计	被引频次：69[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/22517
专题	中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,et al. A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation[J]. ADVANCED FUNCTIONAL MATERIALS,2017,27(28):1700769.
APA	Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,&Chen, Lingxin.(2017).A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation.ADVANCED FUNCTIONAL MATERIALS,27(28),1700769.
MLA	Han, Xiaoyue,et al."A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation".ADVANCED FUNCTIONAL MATERIALS 27.28(2017):1700769.