YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation
Han, Xiaoyue1,2; Song, Xinyu1; Yu, Fabiao1; Chen, Lingxin1
Source PublicationADVANCED FUNCTIONAL MATERIALS
ISSN1616-301X
2017-07-26
Volume27Issue:28Pages:1700769
KeywordInflammation Near-infrared Fluorescent Probes Protective Effects Selenocysteine
MOST Discipline CatalogueChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
DOI10.1002/adfm.201700769
Contribution Rank[Han, Xiaoyue; Song, Xinyu; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Department海岸带环境工程技术研究与发展中心
AbstractSelenocysteine (Sec) is a primary kind of reactive selenium species in cells whose antioxidant roles in a series of liver diseases have been featured. However, it is difficult to determine Sec in living cells and in vivo due to its high reactivity and instability. This work reports a ratiometric near-infrared fluorescent probe (Cy-SS) for qualitative and quantitative determination of Sec in living cells and in vivo. The probe is composed of heptamethine cyanine fluorophore, the response unit bis(2-hydroxyethyl)disulfide, and the liver-targeting moiety D-galactose. Based on a detection mechanism of selenium-sulfur exchange reaction, the concentrations of Sec in HepG2, HL-7702 cells, and primary mouse hepatocytes is determined as 3.08 +/- 0.11 x 10(-6) M, 4.03 +/- 0.16 x 10(-6) M and 4.34 +/- 0.30 x 10(-6) M, respectively. The probe can selectively accumulate in liver. The ratio fluorescence signal of the probe can be employed to quantitatively analyze the fluctuation of Sec concentrations in cells and mice models of acute hepatitis. The experimental results demonstrate that Sec plays important antioxidant and anti-inflammatory roles during inflammatory process. And the levels of intracellular Sec have a close relationship with the degree of liver inflammation. The above imaging detections make this new probe a potential candidate for the accurate diagnosis of inflammation.
Funding OrganizationNational Nature Science Foundation of China(21405172 ; program of Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS(KF2016-22) ; 21575159 ; 21275158)
SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordLIVING CELLS ; THIOREDOXIN REDUCTASE ; CARBON-TETRACHLORIDE ; SELENIUM COMPOUNDS ; LIVER-DAMAGE ; THIOLS ; SULFUR ; MECHANISMS ; TISSUES ; RATS
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS IDWOS:000406183100007
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/22517
Collection中科院海岸带环境过程与生态修复重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,et al. A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation[J]. ADVANCED FUNCTIONAL MATERIALS,2017,27(28):1700769.
APA Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,&Chen, Lingxin.(2017).A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation.ADVANCED FUNCTIONAL MATERIALS,27(28),1700769.
MLA Han, Xiaoyue,et al."A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation".ADVANCED FUNCTIONAL MATERIALS 27.28(2017):1700769.
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