A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation
Han, Xiaoyue1,2; Song, Xinyu1; Yu, Fabiao1; Chen, Lingxin1
2017-07-26
发表期刊ADVANCED FUNCTIONAL MATERIALS
ISSN1616-301X
卷号27期号:28
摘要Selenocysteine (Sec) is a primary kind of reactive selenium species in cells whose antioxidant roles in a series of liver diseases have been featured. However, it is difficult to determine Sec in living cells and in vivo due to its high reactivity and instability. This work reports a ratiometric near-infrared fluorescent probe (Cy-SS) for qualitative and quantitative determination of Sec in living cells and in vivo. The probe is composed of heptamethine cyanine fluorophore, the response unit bis(2-hydroxyethyl)disulfide, and the liver-targeting moiety D-galactose. Based on a detection mechanism of selenium-sulfur exchange reaction, the concentrations of Sec in HepG2, HL-7702 cells, and primary mouse hepatocytes is determined as 3.08 +/- 0.11 x 10(-6) M, 4.03 +/- 0.16 x 10(-6) M and 4.34 +/- 0.30 x 10(-6) M, respectively. The probe can selectively accumulate in liver. The ratio fluorescence signal of the probe can be employed to quantitatively analyze the fluctuation of Sec concentrations in cells and mice models of acute hepatitis. The experimental results demonstrate that Sec plays important antioxidant and anti-inflammatory roles during inflammatory process. And the levels of intracellular Sec have a close relationship with the degree of liver inflammation. The above imaging detections make this new probe a potential candidate for the accurate diagnosis of inflammation.
关键词Inflammation Near-infrared Fluorescent Probes Protective Effects Selenocysteine
DOI10.1002/adfm.201700769
项目资助者National Nature Science Foundation of China(21405172 ; program of Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS(KF2016-22) ; 21575159 ; 21275158)
收录类别SCI
关键词[WOS]LIVING CELLS ; THIOREDOXIN REDUCTASE ; CARBON-TETRACHLORIDE ; SELENIUM COMPOUNDS ; LIVER-DAMAGE ; THIOLS ; SULFUR ; MECHANISMS ; TISSUES ; RATS
文章类型Article
语种英语
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS记录号WOS:000406183100007
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/22517
专题中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,et al. A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation[J]. ADVANCED FUNCTIONAL MATERIALS,2017,27(28).
APA Han, Xiaoyue,Song, Xinyu,Yu, Fabiao,&Chen, Lingxin.(2017).A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation.ADVANCED FUNCTIONAL MATERIALS,27(28).
MLA Han, Xiaoyue,et al."A Ratiometric Near-Infrared Fluorescent Probe for Quantification and Evaluation of Selenocysteine-Protective Effects in Acute Inflammation".ADVANCED FUNCTIONAL MATERIALS 27.28(2017).
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