A preparation method of phycocyanin polypeptide, including the following steps : adding water to phycocyanin, stirring to fully dissolve it, and obtaining a phycocyanin solution; Add papain or neutral protease to phycocyanin solution, stir well, sonicate for 30~50min to obtain enzymatic hydrolysate; The enzymatic hydrolysate is heated to 85 °C ~ 95 °C and kept warm for 5~15min, cooled after killing the enzyme, centrifuged, and the supernatant is taken as the enzymatic hydrolysate; The enzymatic hydrolysate was ultrafiltered to obtain ultrafiltrate; The ultrafiltrate was vacuum concentrated and spray-dried to obtain phycocyanin polypeptide. The preparation method of the above phycocyanin polypeptide adopts ultrasound-assisted enzymatic hydrolysis, ultrasonic waves have cavitation effect, and the bubbles produced by extrusion rupture can instantly produce extremely strong mechanical shear force, so that the protein degrades or changes the protein conformation, promotes its hydrophilic groups to be exposed more, improves the solubility of phycocyanin in water, is conducive to the combination of enzymes and phycocyanin substrate, and can significantly improve the enzymatic hydrolysis efficiency of phycocyanin.
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