| Diversity of Cultivable Protease-Producing Bacteria in Laizhou Bay Sediments, Bohai Sea, China | |
Li, Yan ; Wu, Chaoya; Zhou, Mingyang; Wang, En Tao; Zhang, Zhenpeng; Liu, Wei; Ning, Jicai; Xie, Zhihong; Xie, ZH (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China. zhxie@yic.ac.cn
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| Source Publication | FRONTIERS IN MICROBIOLOGY
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| ISSN | 1664-302X |
| 2017-03-16 | |
| Volume | 8Pages:405 |
| Keyword | Community Pseudoalteromonas Discharge |
| DOI | 10.3389/fmicb.2017.00405 |
| Contribution Rank | [Li, Yan; Wu, Chaoya; Zhang, Zhenpeng; Liu, Wei; Ning, Jicai; Xie, Zhihong] Chinese Acad Sci, Key Lab Coastal Biol & Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Wu, Chaoya] Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou, Peoples R China; [Zhou, Mingyang] Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan, Peoples R China; [Wang, En Tao] Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico |
| Department | 海岸带生物学与生物资源利用所重点实验室 |
| Abstract | Protease-producing bacteria are widespread in ocean sediments and play important roles in degrading sedimentary nitrogenous organic materials. However, the diversity of the bacteria and the proteases involved in such processes remain largely unknown especially for communities in enclosed sea bays. Here, we investigated the diversity of the extracellular protease-producing bacteria and their protease types in Laizhou Bay. A total of 121 bacterial isolates were obtained from sediment samples in 7 sites and their protease types were characterized. The abundance of cultivable protease producing bacteria was about 104 CFU g(-1) of sediment. Phylogenetic analysis based on 16S rRNA gene sequences suggest that the isolates belonged to 17 genera from 4 phyla including Firmicutes, Actiriobacteria, Proteobacteria and Bacteroidetes, and mainly dominated by the genera Pseudoalteromonas(40.5%), Bacillus(36.3%), and Photobacterium(5.8%). The diversity and community structure varied among different sampling sites but no significant correlation was observed with soil sediment's characteristics. Enzyme activity and inhibition tests further revealed that all isolates secreted proteases that were inhibited by serine and/or metalloprotease inhibitors, and a smaller proportion was inhibited by inhibitors of cysteine and/or aspartic proteases. Furthermore, all isolates effectively degraded casein and/or gelatin with only a few that could hydrolyze elastin, suggesting that the bacteria were producing different kinds of serine proteases or metalloproteases. This study provided novel insights on the community structure of cultivable protease-producing bacteria near the Yellow River estuary of an enclosed sea bay. |
| Subtype | Article |
| Funding Organization | Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020403] ; National Natural Science Foundation of China [31600009, 31370108, 31570063] ; Key Research Program of the Chinese Academy of Sciences [KZZD-EW-14] ; One Hundred-Talent Plan of Chinese Academy of Sciences (CAS) ; Shandong Independent Innovation and Achievement Transformation Program [2014ZZCX07303] ; IPN, Mexico [SIP 20150597, 20160883] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | COMMUNITY ; PSEUDOALTEROMONAS ; DISCHARGE |
| WOS Research Area | Microbiology |
| WOS ID | WOS:000396345300001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/22014 |
| Collection | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带信息集成与战略规划研究中心 |
| Corresponding Author | Xie, ZH (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China. zhxie@yic.ac.cn |
| Affiliation | 1.Chinese Acad Sci, Key Lab Coastal Biol & Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 2.Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou, Peoples R China 3.Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou, Peoples R China 4.Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan, Peoples R China 5.Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico |
| Recommended Citation GB/T 7714 | Li, Yan,Wu, Chaoya,Zhou, Mingyang,et al. Diversity of Cultivable Protease-Producing Bacteria in Laizhou Bay Sediments, Bohai Sea, China[J]. FRONTIERS IN MICROBIOLOGY,2017,8:405. |
| APA | Li, Yan.,Wu, Chaoya.,Zhou, Mingyang.,Wang, En Tao.,Zhang, Zhenpeng.,...&Xie, ZH .(2017).Diversity of Cultivable Protease-Producing Bacteria in Laizhou Bay Sediments, Bohai Sea, China.FRONTIERS IN MICROBIOLOGY,8,405. |
| MLA | Li, Yan,et al."Diversity of Cultivable Protease-Producing Bacteria in Laizhou Bay Sediments, Bohai Sea, China".FRONTIERS IN MICROBIOLOGY 8(2017):405. |
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| Diversity of Cultiva（1012KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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