| Diversity of Cultivable Protease-Producing Bacteria in Laizhou Bay Sediments, Bohai Sea, China | |
Li, Yan ; Wu, Chaoya; Zhou, Mingyang; Wang, En Tao; Zhang, Zhenpeng; Liu, Wei; Ning, Jicai; Xie, Zhihong; Xie, ZH (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China. zhxie@yic.ac.cn
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| 发表期刊 | FRONTIERS IN MICROBIOLOGY
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| ISSN | 1664-302X |
| 2017-03-16 | |
| 卷号 | 8页码:405 |
| 关键词 | Community Pseudoalteromonas Discharge |
| DOI | 10.3389/fmicb.2017.00405 |
| 产权排序 | [Li, Yan; Wu, Chaoya; Zhang, Zhenpeng; Liu, Wei; Ning, Jicai; Xie, Zhihong] Chinese Acad Sci, Key Lab Coastal Biol & Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Wu, Chaoya] Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou, Peoples R China; [Zhou, Mingyang] Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan, Peoples R China; [Wang, En Tao] Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Protease-producing bacteria are widespread in ocean sediments and play important roles in degrading sedimentary nitrogenous organic materials. However, the diversity of the bacteria and the proteases involved in such processes remain largely unknown especially for communities in enclosed sea bays. Here, we investigated the diversity of the extracellular protease-producing bacteria and their protease types in Laizhou Bay. A total of 121 bacterial isolates were obtained from sediment samples in 7 sites and their protease types were characterized. The abundance of cultivable protease producing bacteria was about 104 CFU g(-1) of sediment. Phylogenetic analysis based on 16S rRNA gene sequences suggest that the isolates belonged to 17 genera from 4 phyla including Firmicutes, Actiriobacteria, Proteobacteria and Bacteroidetes, and mainly dominated by the genera Pseudoalteromonas(40.5%), Bacillus(36.3%), and Photobacterium(5.8%). The diversity and community structure varied among different sampling sites but no significant correlation was observed with soil sediment's characteristics. Enzyme activity and inhibition tests further revealed that all isolates secreted proteases that were inhibited by serine and/or metalloprotease inhibitors, and a smaller proportion was inhibited by inhibitors of cysteine and/or aspartic proteases. Furthermore, all isolates effectively degraded casein and/or gelatin with only a few that could hydrolyze elastin, suggesting that the bacteria were producing different kinds of serine proteases or metalloproteases. This study provided novel insights on the community structure of cultivable protease-producing bacteria near the Yellow River estuary of an enclosed sea bay. |
| 文章类型 | Article |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020403] ; National Natural Science Foundation of China [31600009, 31370108, 31570063] ; Key Research Program of the Chinese Academy of Sciences [KZZD-EW-14] ; One Hundred-Talent Plan of Chinese Academy of Sciences (CAS) ; Shandong Independent Innovation and Achievement Transformation Program [2014ZZCX07303] ; IPN, Mexico [SIP 20150597, 20160883] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | COMMUNITY ; PSEUDOALTEROMONAS ; DISCHARGE |
| 研究领域[WOS] | Microbiology |
| WOS记录号 | WOS:000396345300001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/22014 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带信息集成与战略规划研究中心 |
| 通讯作者 | Xie, ZH (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China. zhxie@yic.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Biol & Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 2.Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou, Peoples R China 3.Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou, Peoples R China 4.Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan, Peoples R China 5.Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico |
| 推荐引用方式 GB/T 7714 | Li, Yan,Wu, Chaoya,Zhou, Mingyang,et al. Diversity of Cultivable Protease-Producing Bacteria in Laizhou Bay Sediments, Bohai Sea, China[J]. FRONTIERS IN MICROBIOLOGY,2017,8:405. |
| APA | Li, Yan.,Wu, Chaoya.,Zhou, Mingyang.,Wang, En Tao.,Zhang, Zhenpeng.,...&Xie, ZH .(2017).Diversity of Cultivable Protease-Producing Bacteria in Laizhou Bay Sediments, Bohai Sea, China.FRONTIERS IN MICROBIOLOGY,8,405. |
| MLA | Li, Yan,et al."Diversity of Cultivable Protease-Producing Bacteria in Laizhou Bay Sediments, Bohai Sea, China".FRONTIERS IN MICROBIOLOGY 8(2017):405. |
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