Biochemical Characterization of 3-Methyl-4-nitrophenol Degradation in Burkholderia sp Strain SJ98
Min, J; Lu, Y; Hu, XK; Zhou, NY; Hu, XK (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China.; Zhou, NY (reprint author), Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200030, Peoples R China. xkhu@yic.ac.cn; ningyi.zhou@sjtu.edu.cn
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
2016-05-25
Volume7Issue:.Pages:791
Keyword2-chloro-4-nitrophenol 3-methyl-4-nitrophenol Burkholderia Sp Strain Sj98 Catabolism Fenitrothion Para-nitrophenol
DOI10.3389/fmicb.2016.00791
Contribution Rank[Min, Jun; Lu, Yang; Hu, Xiaoke] Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Zhou, Ning-Yi] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200030, Peoples R China
Department海岸带生物学与生物资源利用所重点实验室
AbstractSeveral strains have been reported to grow on 3-methyl-4-nitrophenol (3M4NP), the primary breakdown product of the excessively used insecticide fenitrothion. However, the microbial degradation of 3M4NP at molecular and biochemical levels remains unknown. Here, methyl-1,4-benzoquinone (MBQ) and methylhydroquinone (MHQ), rather than catechol proposed previously, were identified as the intermediates before ring cleavage during 3M4NP degradation by Burkholderia sp. strain SJ98. Realtime quantitative PCR analysis indicated that the pnpABA1CDEF cluster involved in para-nitrophenol (PNP) and 2-chloro-4-nitrophenol (2C4NP) catabolism was also likely responsible for 3M4NP degradation in this strain. Purified PNP 4-monooxygenase (PnpA) is able to catalyze the monooxygenation of 3M4NP to MBQ and exhibited an apparent K-m value of 20.3 +/- 2.54 mu M for 3M4NP, and pnpA is absolutely necessary for the catabolism of 3M4NP by gene knock-out and complementation. PnpB, a 1,4-benzoquinone reductase catalyzes the reduction of MBQ to MHQ, and also found to enhance PnpA activity in vitro in the conversion of 3M4NP to MBQ. By sequential catalysis assays, PnpCD, PnpE, and PnpF were likely involved in the lower pathway of 3M4NP catabolism. Although NpcCD, NpcE, and NpcF are able to catalyze the sequential conversion of MHQ in vitro, these enzymes are unlikely involved in 3M4NP catabolism because their coding genes were not upregulated by 3M4NP induction in vivo. These results revealed that the enzymes involved in PNP and 2C4NP catabolism were also responsible for 3M4NP degradation in strain SJ98. This fills a gap in our understanding of the microbial degradation of 3M4NP at molecular and biochemical levels and also provides another example to illustrate the adaptive flexibility in microbial catabolism for structurally similar compounds.
SubtypeArticle
Indexed BySCI
WOS Keyword2-chloro-4-nitrophenol ; 3-methyl-4-nitrophenol ; Burkholderia sp strain SJ98 ; catabolism ; fenitrothion ; para-nitrophenol
WOS Research AreaMicrobiology
WOS IDWOS:000376484700001
Funding OrganizationYantai Science and Technology Project [2014ZH088] ; National Key Basic Research Program of China (973 Program) [201203725202] ; National Natural Science Foundation of China [41376138]
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/17449
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Corresponding AuthorHu, XK (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China.; Zhou, NY (reprint author), Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200030, Peoples R China. xkhu@yic.ac.cn; ningyi.zhou@sjtu.edu.cn
Affiliation1.Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res
2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab
Recommended Citation
GB/T 7714
Min, J,Lu, Y,Hu, XK,et al. Biochemical Characterization of 3-Methyl-4-nitrophenol Degradation in Burkholderia sp Strain SJ98[J]. FRONTIERS IN MICROBIOLOGY,2016,7(.):791.
APA Min, J.,Lu, Y.,Hu, XK.,Zhou, NY.,Hu, XK .,...&ningyi.zhou@sjtu.edu.cn.(2016).Biochemical Characterization of 3-Methyl-4-nitrophenol Degradation in Burkholderia sp Strain SJ98.FRONTIERS IN MICROBIOLOGY,7(.),791.
MLA Min, J,et al."Biochemical Characterization of 3-Methyl-4-nitrophenol Degradation in Burkholderia sp Strain SJ98".FRONTIERS IN MICROBIOLOGY 7..(2016):791.
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