| 土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响 |
| 其他题名 | Effects of Oxytetracycline on Population Growth and Genetic Diversity of Euplotesvannus (Marine Protist)
|
| 付娆; 龚骏 ; 张倩倩; 龚骏,中国科学院烟台海岸带研究所 微生物生态与物质循环实验室,E-mail：jgong@yic.ac.cn
|
| 发表期刊 | 中国科学:生命科学
 |
| ISSN | 1674-7232
|
| 2016-09-20
|
| 卷号 | 46期号:9页码:1113-1120 |
| 关键词 | 扇形游仆虫
抗生素
污染物
种群生长
单细胞基因组分析
遗传多样性
|
| DOI | 10.1360/N052016-00058
|
| 产权排序 | 中国科学院烟台海岸带研究所微生物生态与物质循环实验室; |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室
|
| 英文摘要 | 抗生素作为一类环境污染物在海岸带生境中广泛存在,它们对海洋原生生物遗传多样性的影响还不清楚.本工作以一种海洋纤毛虫原生生物(扇形游仆虫(Euplotes vannus))为例,研究了一种常见四环类抗生素(土霉素)对其生长的影响,并通过单细胞(个体)分析,探索了抗生素对其大核基因组中核糖体小亚基RNA基因(SSU r DNA)序列多样性的影响.结果表明,与对照组相比,3个土霉素处理组(1,10和20μg/m L)中纤毛虫的自然增长率随土霉素浓度升高显著降低,细胞大小则呈增大趋势.SSU r DNA序列单倍型多样性、核苷酸多样性和GC含量随抗生素浓度升高呈现降低趋势.序列单核苷酸多态性位点的碱基转... |
| 中文摘要 | In order to understand the potential impact of antibiotic pollutants on marine protists, the marine ciliate Euplotes
vannus was selected as a model organism to test the effects of oxytetracycline (OTC), a tetracycline antibiotic widely
used and distributed in coastal environments. The effects of OTC on growth, cell size, and small subunit ribosomal
DNA (SSU rDNA) sequence polymorphisms were documented based on single-cell analysis. For the three OTC
treatments used (concentrations of 1, 10, and 20 µg/mL), the growth rate decreased significantly with increasing
OTC concentrations, whereas cell size showed a tendency of increase, compared with the control. SSU rDNA of
single cells generally exhibited lower haplotype and nucleotide diversity, along with slightly lower GC content at
higher OTC concentrations. For single nucleotide polymorphisms in SSU rDNA sequences, the frequency of
transition was much higher than that of transversion in both control and OTC treatments, although the frequency of
transversion increased with increasing dose of OTC. In the treatment with the highest OTC dose, T to C transversion
occurred more frequently and A to G reduced substantially, compared with the control. This study indicates that
antibiotic pollution has a significant impact on phenotype and physiology of marine ciliates and can lead to changes
in the genetic structure of genomic rDNA. It also highlights the high plasticity of protist genomes, which allows
these unicellular eukaryotes to rapidly adapt to antibiotic stress. |
| 文章类型 | 期刊论文
|
| 资助机构 | 国家自然科学基金(批准号:41176143)
; 山东省杰出青年基金项目(批准号:JQ201210)资助
|
| 收录类别 | CSCD
|
| 语种 | 中文
|
| CSCD记录号 | CSCD:5812037
|
| 引用统计 |
|
| 文献类型 | 期刊论文
|
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/17285
|
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
|
| 通讯作者 | 龚骏,中国科学院烟台海岸带研究所 微生物生态与物质循环实验室,E-mail：jgong@yic.ac.cn |
| 作者单位 | 中国科学院烟台海岸带研究所微生物生态与物质循环实验室
|
推荐引用方式
GB/T 7714 |
付娆,龚骏,张倩倩,等. 土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响[J]. 中国科学:生命科学,2016,46(9):1113-1120.
|
| APA |
付娆,龚骏,张倩倩,&龚骏,中国科学院烟台海岸带研究所 微生物生态与物质循环实验室,E-mail：jgong@yic.ac.cn.(2016).土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响.中国科学:生命科学,46(9),1113-1120.
|
| MLA |
付娆,et al."土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响".中国科学:生命科学 46.9(2016):1113-1120.
|
修改评论